Cho Kang Jun, Park Eun Young, Kim Hyo Sin, Koh Jun Sung, Kim Joon Chul
Department of Urology, Bucheon St. Mary's Hospital, The Catholic University of Korea, 327 Sosa-Ro, Wonmi-gu, Bucheon, 420-717, Gyeonggi-do, Korea.
World J Urol. 2014 Jun;32(3):677-82. doi: 10.1007/s00345-013-1099-y. Epub 2013 May 23.
To investigate transient receptor potential vanilloid 4 (TRPV4) expression and the effects of ruthenium red (RR)-TRPV antagonist-on detrusor overactivity (DO) associated with bladder outlet obstruction (BOO).
Rats were randomly assigned to 3 groups. The control group (n = 10) included sham-operated rats. The BOO-group without RR (n = 15) and BOO-group with RR (n = 15) underwent partial BOO surgery. Three weeks postoperatively, cystometrography was performed in all rats. After confirming DO, RR was instilled intravesically in the BOO-group with RR. Urodynamic parameters were investigated, including contraction interval (CI) and contraction pressure (CP). TRPV4 expression was evaluated through immunofluorescence staining and western blotting.
The BOO-group without RR had significantly shorter CI and significantly higher CP compared to the control. In the BOO-group with RR, CI was significantly longer compared to the BOO-group without RR. However, change in CP between BOO-group without and with RR was not significantly different. Immunofluorescence staining showed that TRPV4 was localized in the urothelium and detrusor muscles. TRPV4 immunofluorescence signals were increased in the urothelium and detrusor muscle in BOO-group without RR, compared with the control. In western blot analysis, immunoreactive bands indicating expression of TRPV4 were detected in the urothelium and detrusor muscle, and those were significantly increased in the BOO-group without RR compared with the control in the urothelium and detrusor muscle.
TRPV4 plays an important role in the pathophysiology of DO, and RR has a beneficial effect on DO associated with BOO.
研究瞬时受体电位香草酸亚型4（TRPV4）的表达以及钌红（RR）-TRPV拮抗剂对膀胱出口梗阻（BOO）相关逼尿肌过度活动（DO）的影响。
将大鼠随机分为3组。对照组（n = 10）包括假手术大鼠。未用RR的BOO组（n = 15）和用RR的BOO组（n = 15）接受部分BOO手术。术后3周，对所有大鼠进行膀胱测压。确认存在DO后，向用RR的BOO组膀胱内注入RR。研究尿动力学参数，包括收缩间隔（CI）和收缩压（CP）。通过免疫荧光染色和蛋白质印迹法评估TRPV4的表达。
与对照组相比，未用RR的BOO组CI明显缩短，CP明显升高。在用RR的BOO组中，CI比未用RR的BOO组明显延长。然而，未用RR和用RR的BOO组之间CP的变化无显著差异。免疫荧光染色显示TRPV4定位于尿路上皮和逼尿肌。与对照组相比，未用RR的BOO组尿路上皮和逼尿肌中的TRPV4免疫荧光信号增加。在蛋白质印迹分析中，在尿路上皮和逼尿肌中检测到指示TRPV4表达的免疫反应条带，与对照组相比，未用RR的BOO组尿路上皮和逼尿肌中的这些条带明显增加。
TRPV4在DO的病理生理学中起重要作用，RR对与BOO相关的DO有有益作用。
