Oosthuizen C J, Herbert J S, Vermaak L K, Brusnicky J, Fricke J, du Plessis L, Retief A E
Department of Human Genetics, Tygerberg Hospital, Republic of South Africa.
Hum Genet. 1990 Jul;85(2):205-10. doi: 10.1007/BF00193197.
Thirty-nine recombinants isolated from a Y chromosome-specific library were deletion mapped. Seven deletion intervals were defined by hybridization of probes to DNA of eight individuals with aberrant Y chromosomes. Extreme cytogenetic limits of the deletion intervals were determined by in situ hybridization of one probe per deletion interval. Five intervals, with a total of twenty-five probes, were allocated to the long-arm euchromatic region. The probes described will be useful for characterization of aberrant Y chromosomes, in searching for expressed sequences on the Y chromosome, and for further study of the evolutionary relationship between the Y chromosome and other chromosomes.
从一个Y染色体特异性文库中分离出的39个重组体进行了缺失定位。通过将探针与8个具有异常Y染色体个体的DNA杂交，确定了7个缺失区间。每个缺失区间通过一个探针的原位杂交确定了缺失区间的极端细胞遗传学界限。5个区间，共25个探针，被分配到长臂常染色质区域。所描述的探针将有助于异常Y染色体的特征分析、在Y染色体上寻找表达序列以及进一步研究Y染色体与其他染色体之间的进化关系。
