Dasgupta S C, Gomes A, Gomes A, Basu A, Lahiri S C
Department of Pharmacology, School of Tropical Medicine, Calcutta, India.
Indian J Exp Biol. 1990 Feb;28(2):144-8.
Toxin-Hb, a lethal toxic antigenic protein, isolated from the venom of H. bengalensis by CM-cellulose ion-exchange chromatography was a heat labile basic protein with a molecular weight of 10 kDa. It produced irreversible blockade on the isolated rat phrenic nerve diaphragm and chick biventer cervicis. LD50 of toxin Hb was 0.48 mg/kg (iv) in mice. Antiserum was raised in mice by hyperimmunization against toxin Hb. Antitoxin Hb antiserum was immunologically potent as revealed by immunogel-diffusion and immunoelectrophoresis. Five fold protection against the lethal action of toxin Hb was achieved by the antiserum. It also effectively antagonised toxin Hb induced neuromuscular blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations.
毒素-Hb是一种致命的有毒抗原蛋白，通过CM-纤维素离子交换色谱法从孟加拉眼镜蛇毒液中分离得到，是一种分子量为10 kDa的热不稳定碱性蛋白。它对离体大鼠膈神经膈肌和鸡双侧颈肌产生不可逆的阻断作用。毒素Hb在小鼠体内的静脉注射半数致死量为0.48 mg/kg。通过对毒素Hb进行超免疫在小鼠体内制备抗血清。免疫凝胶扩散和免疫电泳显示，抗毒素Hb血清具有免疫活性。该抗血清对毒素Hb的致死作用提供了五倍的保护。它还能有效拮抗毒素Hb对离体大鼠膈神经膈肌和鸡双侧颈肌标本诱导的神经肌肉阻断作用。
