China National Tobacco Quality Supervision and Test Center , No. 2 Fengyang Street, Zhengzhou High and New Technology Industries Development Zone, Zhengzhou 450001, China.
J Agric Food Chem. 2013 Jun 19;61(24):5746-57. doi: 10.1021/jf400887x. Epub 2013 Jun 6.
A multiresidue gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for the analysis of 159 multiclass pesticides in tobacco. A modified QuEChERS sample preparation technique, based on acetonitrile extraction and toluene dilution, followed by dispersive solid-phase extraction (d-SPE) cleanup using primary-secondary amine (PSA) and octadecyl (C18) sorbents, was used for sample treatment. Key performance parameters investigated were linearity, recovery, relative standard deviation (RSD), limit of detection, and limit of quantitation. With the exception of chinomethionate and folpet, recoveries for pesticides ranged from 69 to 141%, and the RSDs ranged from 2 to 27%. The validated method was applied to the analysis of 118 real samples, and positive results were obtained for 116 samples, with 25 different pesticides being detected.
建立了一种多残留气相色谱-串联质谱（GC-MS/MS）方法，用于分析烟草中的 159 种多类农药。采用改良的 QuEChERS 样品前处理技术，以乙腈提取和甲苯稀释，然后使用聚酰胺（PSA）和十八烷基（C18）吸附剂进行分散固相萃取（d-SPE）净化，用于样品处理。研究了线性、回收率、相对标准偏差（RSD）、检出限和定量限等关键性能参数。除了灭蝇胺和灭菌丹外，农药的回收率在 69%至 141%之间，RSD 在 2%至 27%之间。经验证的方法应用于 118 个实际样品的分析，116 个样品呈阳性，检测到 25 种不同的农药。
