Paul Anindita, Majumder Sujan, Prasad L K, Madhav M Sheshu, Johnson Nalli, Padmaja K, Singh Satyapriya
Division of Post harvest & Value Addition, ICAR- National Institute for Research on Commercial Agriculture, Rajahmundry, Andhra Pradesh, India.
ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, India.
PLoS One. 2025 Aug 28;20(8):e0328446. doi: 10.1371/journal.pone.0328446. eCollection 2025.
Pendimethalin is generally used as pre-emergence herbicide cum suckericide in different growing region of Flue-Cured Verginia (FCV) tobacco by applying indiscriminately in irrigation channel. In quest of safety regulations related to pendimethalin residues in cured leaf tobacco and tobacco growing soils, a rapid and robust method is developed by GC-MS single quadruple system.. The GC-MS single ion monitoring (SIM) analytical method, achieved good linearity (R2 > 0.99) with LOD and LOQ values of 0.001 mg/kg and 0.005 mg/kg, respectively. The method gave more than 80% recovery (5% RSD) showed compliance with international specification of DG-SANTE guidelines. In the majority of samples, pesticide residue levels were below the guidance residue levels (GRL; for pendimethalin GRL 5 ppm) value set by Center for Scientific Research Relative to Tobacco (CORESTA). The analytical method developed for true detection of pendimethalin residue at very trace levels with acceptable recovery level and matrix effect. The method is improved in terms of sentivity and precision as per regulatory norms of exporting commercial crops like tobacco. Looking at the consumer safety the method can be used in monitoring the pendimethalin residues in FCV tobacco and FCV tobacco grown soils at regular intervals.
二甲戊灵通常作为芽前除草剂兼杀芽剂，通过在灌溉渠道中不加区分地施用，用于弗吉尼亚烤烟（FCV）烟草的不同种植区域。为了寻求与烤烟叶片和烟草种植土壤中二甲戊灵残留相关的安全法规，采用气相色谱 - 质谱单四极杆系统开发了一种快速且可靠的方法。气相色谱 - 质谱单离子监测（SIM）分析方法具有良好的线性（R2>0.99），检测限（LOD）和定量限（LOQ）分别为0.001mg/kg和0.005mg/kg。该方法回收率超过80%（相对标准偏差5%），符合DG - SANTE指南的国际规范。在大多数样品中，农药残留水平低于烟草科研中心（CORESTA）设定的指导残留水平（GRL；二甲戊灵的GRL为5ppm）值。所开发的分析方法能够在可接受的回收率水平和基质效应下，真正检测到痕量水平的二甲戊灵残留。根据出口烟草等商业作物的监管规范，该方法在灵敏度和精密度方面得到了改进。从消费者安全角度看，该方法可用于定期监测FCV烟草及其种植土壤中的二甲戊灵残留。
