16,16-二甲基前列腺素E2和/或同基因骨髓移植可提高小鼠在超致死剂量全身照射后的存活率。

16,16-Dimethyl prostaglandin E2 and/or syngeneic bone marrow transplantation increase mouse survival after supra-lethal total body irradiation.

作者信息

Berk L B, Patrene K D, Boggs S S

机构信息

Department of Radiation Oncology, University of Pittsburgh School of Medicine, PA 15261.

出版信息

Int J Radiat Oncol Biol Phys. 1990 Jun;18(6):1387-92. doi: 10.1016/0360-3016(90)90312-8.

DOI:10.1016/0360-3016(90)90312-8

PMID:2370188

Abstract

We evaluated the effects of 16,16-dimethyl prostaglandin E2 (dm-PGE2), with and without syngeneic bone marrow transplantation (BMT) on the survival and hematopoietic recovery of mice given 14-20 Gy total body irradiation (TBI). Survival of mice given combined dm-PGE2 and BMT was improved significantly over that of mice given either treatment alone. The 30-day survival after 14, 15, 16 or 18 Gy TBI for combined treatment was 97, 90, 20 or 10 percent, respectively. The corresponding 30-day survival rates for mice given BMT alone were 69, 60, 7 or 0 percent, respectively. For dm-PGE2 alone, 30-day survival was 63, 20, 10 or 0 percent, respectively. Deaths in both dm-PGE2 treated groups generally occurred after day 10 whereas deaths in the BMT group occurred before day 10. All irradiated controls were dead on or before day 10; after larger doses, deaths clustered around day 5. After 20 Gy TBI, all mice in all groups were dead by day 7. Studies of white blood cell recovery 1-9 days after 14 Gy TBI showed improvement with BMT, whereas dm-PGE2 did not enhance recovery. Nucleated cells per humerus, spleen weight, and spleen iron uptake (erythropoiesis) were also improved by BMT but not dm-PGE2.

摘要

我们评估了16,16 - 二甲基前列腺素E2（dm - PGE2）在有或无同基因骨髓移植（BMT）情况下，对接受14 - 20 Gy全身照射（TBI）小鼠的存活及造血恢复的影响。联合使用dm - PGE2和BMT的小鼠存活率显著高于单独使用任何一种治疗的小鼠。在接受14、15、16或18 Gy TBI后，联合治疗组的30天存活率分别为97%、90%、20%或10%。单独接受BMT的小鼠相应的30天存活率分别为69%、60%、7%或0%。单独使用dm - PGE2时，30天存活率分别为63%、20%、10%或0%。两个dm - PGE2治疗组的死亡通常发生在第10天之后，而BMT组的死亡发生在第10天之前。所有受照射的对照组在第10天或之前死亡；剂量较大时，死亡集中在第5天左右。在20 Gy TBI后，所有组的所有小鼠在第7天死亡。对14 Gy TBI后1 - 9天白细胞恢复情况的研究表明，BMT可促进恢复，而dm - PGE2不能增强恢复。BMT还可改善每只肱骨的有核细胞数、脾脏重量及脾脏铁摄取（红细胞生成），但dm - PGE2无此作用。