Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
Int J Environ Res Public Health. 2013 May 23;10(6):2109-19. doi: 10.3390/ijerph10062109.
The purpose of this study was to investigate the oxidative damage induced by dietary nickel chloride (NiCl2) in the intestinal mucosa of different parts of the intestine of broilers, including duodenum, jejunum and ileum. A total of 240 one-day-old broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600 or 900 mg/kg NiCl2 during a 42-day experimental period. The results showed that the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and the ability to inhibit hydroxy radical and glutathione (GSH) content were significantly (p < 0.05 or p < 0.01) decreased in the 300, 600 and 900 mg/kg groups in comparison with those of the control group. In contrast, malondialdehyde (MDA) content was significantly (p < 0.05 or p < 0.01) higher in the 300, 600 and 900 mg/kg groups than that in the control group. It was concluded that dietary NiCl2 in excess of 300 mg/kg could cause oxidative damage in the intestinal mucosa in broilers, which finally impaired the intestinal functions including absorptive function and mucosal immune function. The oxidative damage might be a main mechanism on the effects of NiCl2 on the intestinal health of broilers.
本研究旨在探讨日粮氯化镍（NiCl2）对肉鸡不同肠段（十二指肠、空肠和回肠）肠黏膜氧化损伤的影响。将 240 只 1 日龄肉鸡随机分为 4 组，分别饲喂基础玉米-豆粕日粮（对照组）或添加 300、600 或 900 mg/kg NiCl2 的基础日粮，试验期为 42 天。结果表明，与对照组相比，300、600 和 900 mg/kg 组肉鸡超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性以及羟自由基清除能力和谷胱甘肽（GSH）含量显著（p < 0.05 或 p < 0.01）降低，而丙二醛（MDA）含量显著（p < 0.05 或 p < 0.01）升高。综上可知，日粮中 NiCl2 含量超过 300 mg/kg 可导致肉鸡肠黏膜氧化损伤，进而损害肉鸡的肠道功能，包括吸收功能和黏膜免疫功能。氧化损伤可能是 NiCl2 影响肉鸡肠道健康的主要机制之一。
