双光子比率荧光成像技术用于研究 pH 响应性嵌段共聚物胶束纳米载体的细胞内转运途径。
Two-photon ratiometric fluorescent mapping of intracellular transport pathways of pH-responsive block copolymer micellar nanocarriers.
机构信息
CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China.
出版信息
Adv Healthc Mater. 2013 Dec;2(12):1576-81. doi: 10.1002/adhm.201200436. Epub 2013 May 24.
Ultrasensitive ratiometric fluorescent pH probes based on dual dye-labeled pH-responsive diblock copolymer micellar scaffold are constructed. The pH-sensitive emitting nature of BTPE dyes and emission turn-on of CMA moieties triggered by pH-actuated micelle-to-unimer of diblock scaffold synergistically contribute to the observed ≈250-fold changes of BTPE/CMA emission intensity ratios in the whole pH range. Two-photon ratiometric fluorescent pH mapping of intracellular gradients subjected by pH-responsive micellar nanoparticles in their endocytic pathway has been thus achieved.
基于双染料标记的 pH 响应两亲嵌段共聚物胶束支架构建了超灵敏比率荧光 pH 探针。BTPE 染料的 pH 敏感发射特性和 CMA 部分在 pH 驱动的胶束-齐聚物构象转变协同作用下，使共聚物支架的荧光强度比在整个 pH 范围内发生了约 250 倍的变化。因此，实现了在其内吞途径中 pH 响应胶束纳米粒子的细胞内梯度的双光子比率荧光 pH 成像。
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