Endocrine and Metabolism Research Center, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, I.R. Iran.
IET Nanobiotechnol. 2013 Mar;7(1):1-6. doi: 10.1049/iet-nbt.2012.0001.
Precise detection of 3-hydroxybutyrate (HB) in biological samples is of great importance for management of diabetic patients. In this study, an HB biosensor based on single-walled carbon nanotubes (SWCNTs)-modified screen-printed electrode (SPE) was developed to determine the concentration of HB in serum. The specific detecting enzyme, HB dehydrogenase, was physically immobilised on SWCNTs deposited on the surface of SPEs. The electrochemical measurement of HB that involved cyclic voltammetry was based on the sAgnal produced by j3-nicotinamide adenine dinucleotide (NADH), one of the products of the enzymatic reaction. The application of SWCNT reduced the oxidation potential of NADH to about -0.05 V. Electrochemical measurements showed that the response of this biosensor had relevant good linearity in the range of 0.1-2 mM with a low detection limit of 0.009 mM. Investigation of biosensor response in the presence of interfering molecules verified its specificity. Furthermore, the study of long-term stability demonstrated the acceptable efficiency of this biosensor for about 100 days.
精确检测生物样本中的 3-羟基丁酸（HB）对于糖尿病患者的管理非常重要。在这项研究中，开发了一种基于单壁碳纳米管（SWCNTs）修饰的丝网印刷电极（SPE）的 HB 生物传感器，用于测定血清中 HB 的浓度。特异性检测酶 HB 脱氢酶物理固定在沉积在 SPE 表面的 SWCNTs 上。涉及循环伏安法的 HB 的电化学测量是基于作为酶反应产物之一的 j3-烟酰胺腺嘌呤二核苷酸（NADH）产生的信号。SWCNT 的应用将 NADH 的氧化电位降低到约-0.05 V。电化学测量表明，该生物传感器在 0.1-2 mM 的范围内具有相关的良好线性关系，检测限低至 0.009 mM。在存在干扰分子的情况下对生物传感器响应的研究验证了其特异性。此外，对长期稳定性的研究表明，这种生物传感器在大约 100 天内具有可接受的效率。
