Ono T, Eto K, Sakata Y, Takeda M
J Lab Clin Med. 1975 Jun;85(6):1022-31.
A new colimetric assay for serum monoamine oxidase and clinical findings based on this method are described. This rapid, precise procedure employs 1-[(4-aminomethylphenyl)azol]-2-naphthol as a substrate. The enzymatically generated 1-[(4-formyl phenyl)azo]-2-naphthol is extracted into cyclohexane to form a colored solution whose absorbance is determined at 500 nm. The method proved to be extremely simple, and the results obtained correlated well with those given by the standard method of McEwen.
本文描述了一种新的血清单胺氧化酶比色测定法以及基于该方法的临床研究结果。这种快速、精确的方法使用1-[(4-氨甲基苯基)偶氮]-2-萘酚作为底物。酶促生成的1-[(4-甲酰基苯基)偶氮]-2-萘酚被萃取到环己烷中形成有色溶液，在500nm处测定其吸光度。该方法被证明极其简单，所得结果与麦克尤恩标准方法给出的结果相关性良好。
