Fan Hua-Qian, Shen Zhi-Bin, Chen Yan-Fen, Wu Jie-Ying, Yang Chao-Yan, Liang Wan-Nian, Tang Chun-Ping
Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhong Yao Cai. 2012 Dec;35(12):1981-5.
To examine the antifungal effect of different extract of Dryopteris fragrans (L.) Schott. in vitro, and screen the effective fraction from those extracts.
Separated the Dryopteris fragrans extract and got four parts by refluxing extraction,and determined the contents of total phloroglucinol. Disc agar diffusion method and solid agar dilution method were used to determine inhibitory effect. Minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of different parts of Dryopteris fragrans extract against four strains of common clinical dermatophytes were investigated.
The data showed that the contents sequence of total phloroglucinol was in the following order: 95% -ethanol extract > water extract > diethyl ether extract > petroleum ether extract, and the antimicrobial activities against the four dermatophytes were as following order: 95% -ethanol extract > water extract > di-ethyl ether extract > petroleum ether extract.
The contents of total phloroglucinol in 95% -ethanol extract of Dryopteris fragrans is the highest, and the antifungal activity against dermatophytes in vitro is the strongest. The effective fraction of Dryopteris fragrans is the 95%-ethanol extract.
研究不同提取物对香鳞毛蕨的体外抗真菌作用，并从提取物中筛选有效部位。
采用回流提取法对香鳞毛蕨提取物进行分离，得到4个部位，测定各部位间苯三酚总含量。采用滤纸片琼脂扩散法和固体琼脂稀释法测定抑菌作用。考察香鳞毛蕨提取物不同部位对4种临床常见皮肤癣菌的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。
数据表明，间苯三酚总含量顺序为：95%乙醇提取物＞水提取物＞乙醚提取物＞石油醚提取物，对4种皮肤癣菌的抑菌活性顺序为：95%乙醇提取物＞水提取物＞乙醚提取物＞石油醚提取物。
香鳞毛蕨95%乙醇提取物中间苯三酚总含量最高，体外对皮肤癣菌的抗真菌活性最强。香鳞毛蕨的有效部位为95%乙醇提取物。
