Fang Wei-Jin, Feng Jian-Fang, Lu Xi-Ming, Lv Mao-Ping, Cao Shan-Shan, Li Rui-Fang, Li Yan, Li Xiao-Ming
Laboratory of Pharmacology and Molecular Biology, Medical College, Henan University of Science and Technology,Luoyang 471003, China.
Zhong Yao Cai. 2012 Dec;35(12):1985-9.
To observe the effect of Cornus officinalis fruit core extract on cardiac hypertrophy induced by two kidney two clip (2K2C) and its mechanism.
Male Sprague-Dawley rats were randomly divided into 4 groups: sham-operated group, model group and treatment groups (300, 600 mg/kg). Rats were intragastric administered medicine for 4 weeks from the fourth week after surgery. Sham-operated and 2K2C rats were given vehicle for 4 weeks. Blood pressure and hemodynamic parameters were measured. Left ventricular weight to body weight (LVM/BM) ratio was calculated. Paraffin-embedded hearts were cut into 5 microm slices, which were stained with hematoxylin-eosin (HE) and Masson for morphological analysis; Western-blot analysis was performed to investigate the effects of Cornus officinalis fruit core extract on the expression of P47phox, Nox4 in myocardium.
Compared with sham-operated group, the blood pressure and LVM/BM ratios were markedly elevated in model groups. Meanwhile cardiomyocyte cross sectional areas was markedly increased and myocardial fibers showed disordered arrangement while these parameters were markedly reversed after treatment with Cornus officinalis fruit core extract for 4 weeks. At 8th weeks after operation, model rats developed obvious LV hypertrophy. Cornus officinalis fruit core extract, more significant in high dose, decreased the blood pressure and LVM/BM ratios and reversed the cardiomyocyte hypertrophy and myocardial fibrosis. Moreover, Cornus officinalis fruit core extract decreased the expression of P47phox and Nox4 which elevated in LV in model rats.
Cornus officinalis fruit core extract could significantly decrease the blood pressure, reverse cardiac hypertrophy and improve the function of heart which is possibly associated with the down-regulation of P47phox and Nox4.
观察山茱萸果核提取物对双肾双夹（2K2C）诱导的心脏肥大的影响及其机制。
将雄性Sprague-Dawley大鼠随机分为4组：假手术组、模型组和治疗组（300、600mg/kg）。术后第4周起，大鼠灌胃给药4周。假手术组和2K2C大鼠给予溶媒4周。测量血压和血流动力学参数。计算左心室重量与体重（LVM/BM）比值。将石蜡包埋的心脏切成5微米切片，用苏木精-伊红（HE）和Masson染色进行形态学分析；采用蛋白质免疫印迹法分析山茱萸果核提取物对心肌中P47phox、Nox4表达的影响。
与假手术组相比，模型组血压和LVM/BM比值明显升高。同时，心肌细胞横截面积明显增加，心肌纤维排列紊乱，而山茱萸果核提取物治疗4周后这些参数明显逆转。术后第8周，模型大鼠出现明显的左心室肥大。山茱萸果核提取物，高剂量组作用更显著，可降低血压和LVM/BM比值，逆转心肌细胞肥大和心肌纤维化。此外，山茱萸果核提取物降低了模型大鼠左心室中升高的P47phox和Nox4的表达。
山茱萸果核提取物可显著降低血压，逆转心脏肥大，改善心脏功能，其机制可能与下调P47phox和Nox4有关。
