i-LAB, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China.
Biomaterials. 2013 Aug;34(26):6139-46. doi: 10.1016/j.biomaterials.2013.04.057. Epub 2013 May 21.
Biological studies have shown that collagen/collagen binding domain (CBD)-growth factor composites are effective biomaterials systems for tissue engineering and regeneration. Here we present atomic force spectroscopy (AFM)-based investigations at the single molecule level to address fundamental biophysical questions such as CBD binding sites distribution and the mechanism for controlled release of growth factors from the collagen scaffold. Non-uniformly distributed CBD binding sites on collagen membrane are directly visualized with a quantum dot-based bimodal imaging method. AFM force spectroscopy unbinding experiments reveal that modest unbinding force and dissociation constant of the CBD/collagen interaction could be the key for its successful application in controlled release systems.
生物研究表明，胶原蛋白/胶原蛋白结合域（CBD）-生长因子复合材料是组织工程和再生的有效生物材料系统。在这里，我们在单分子水平上进行基于原子力显微镜（AFM）的研究，以解决基本的生物物理问题，例如 CBD 结合位点的分布以及从胶原蛋白支架中控制释放生长因子的机制。使用基于量子点的双模成像方法可以直接观察到胶原蛋白膜上不均匀分布的 CBD 结合位点。AFM 力谱解吸实验表明，CBD/胶原蛋白相互作用的适度解吸力和离解常数可能是其在控制释放系统中成功应用的关键。
