Sionkowska Alina, Kozłowska Justyna, Lazare Sylvain
Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.
ISM UMR 5255, Université de Bordeaux, 351 Cours de la Libération, F-33405 Talence, France.
Biointerphases. 2014 Jun;9(2):029003. doi: 10.1116/1.4865753.
Collagen was extracted from fish scales (Esox lucius) through demineralization process. Thin films by solvent evaporation from collagen extracted from fish scales were prepared. The surface of thin films made of fish scales collagen was modified by ultraviolet (UV)-irradiation with the wavelength λ = 254 nm. The amino acid composition of the Esox lucius scale collagen was analyzed before and after UV-irradiation by means of high-pressure liquid chromatography. The surface properties of films were investigated using the technique of atomic force microscopy (AFM) and by means of contact angle measurements allowing the calculation of surface free energy. Measurements of the contact angle for diiodomethane (D) and glycerol (G) on the surface of fish collagen films were made and surface free energy was calculated. The structure of collagen before and after UV-irradiation was studied using Fourier-transform infrared spectroscopy. It was found that after UV-irradiation the amount of all amino acids present in collagen molecule decreased. It was found also that the contact angle and the surface free energy were altered by UV-irradiation of collagen film. AFM showed that the surface roughness of collagen films was also altered by UV-irradiation. UV-irradiation caused the decrease of surface roughness due to photochemical processes, which occurred in the top layer of collagen film. The formation of collagen fibrils after solvent evaporation was observed using AFM. The diameter of collagen fibrils was bigger for irradiated collagen film than the diameter of collagen fibrils before UV-irradiation.
通过脱矿质过程从鱼鳞（白斑狗鱼）中提取胶原蛋白。采用溶剂蒸发法，以从鱼鳞中提取的胶原蛋白制备薄膜。用波长λ = 254 nm的紫外线（UV）对鱼鳞胶原蛋白制成的薄膜表面进行改性。采用高压液相色谱法分析紫外线照射前后白斑狗鱼鱼鳞胶原蛋白的氨基酸组成。利用原子力显微镜（AFM）技术并通过接触角测量来研究薄膜的表面性质，从而计算表面自由能。测量二碘甲烷（D）和甘油（G）在鱼胶原蛋白薄膜表面的接触角，并计算表面自由能。利用傅里叶变换红外光谱研究紫外线照射前后胶原蛋白的结构。结果发现，紫外线照射后胶原蛋白分子中所有氨基酸的含量均下降。还发现，紫外线照射会改变胶原蛋白薄膜的接触角和表面自由能。原子力显微镜显示，紫外线照射也会改变胶原蛋白薄膜的表面粗糙度。由于发生在胶原蛋白薄膜顶层的光化学过程，紫外线照射导致表面粗糙度降低。利用原子力显微镜观察了溶剂蒸发后胶原蛋白原纤维的形成。照射后的胶原蛋白薄膜中胶原蛋白原纤维的直径比紫外线照射前的胶原蛋白原纤维直径更大。
