McFedries Amanda, Schwaid Adam, Saghatelian Alan
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Chem Biol. 2013 May 23;20(5):667-73. doi: 10.1016/j.chembiol.2013.04.008.
Understanding the interactions between small molecules and proteins can be approached from different perspectives and is important for the advancement of basic science and drug development. Chemists often use bioactive small molecules, such as natural products or synthetic compounds, as probes to identify therapeutically relevant protein targets. Biochemists and biologists often begin with a specific protein and seek to identify the endogenous metabolites that bind to it. These interests have led to the development of methodology that relies heavily on synthetic and analytical chemistry to identify protein-small molecule and protein-metabolite interactions. Here, we survey these strategies, highlighting key findings, to demonstrate the value of these approaches in answering important chemical and biological questions.
从小分子与蛋白质之间的相互作用可以从不同角度进行研究，这对于基础科学的进步和药物开发都很重要。化学家经常使用生物活性小分子，如天然产物或合成化合物，作为探针来识别具有治疗意义的蛋白质靶点。生物化学家和生物学家通常从一种特定的蛋白质入手，试图识别与之结合的内源性代谢物。这些兴趣推动了主要依赖合成化学和分析化学来识别蛋白质-小分子和蛋白质-代谢物相互作用的方法学的发展。在这里，我们综述这些策略，突出关键发现，以展示这些方法在回答重要化学和生物学问题方面的价值。
