Grassi D, Lagunas N, Amorin M, Pinos H, Panzica G C, Garcia-Segura L M, Collado P
Universidad Nacional de Educacion a Distancia (UNED), Department of Psychobiology, Calle Juan del Rosal 10, 28040 Madrid, Spain; Instituto Cajal, CSIC, Avenida Doctor Arce 37, 28002 Madrid, Spain; University of Turin, Department of Neuroscience, Corso Massimo D'Azeglio 52, 10126 Turin, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), Regione Gonzole 10, 10043 Orbassano, Italy.
Universidad Nacional de Educacion a Distancia (UNED), Department of Psychobiology, Calle Juan del Rosal 10, 28040 Madrid, Spain; Instituto Cajal, CSIC, Avenida Doctor Arce 37, 28002 Madrid, Spain.
Neuroscience. 2013 Sep 17;248:127-35. doi: 10.1016/j.neuroscience.2013.05.024. Epub 2013 May 22.
Estrogen receptors (ERs) α and β are involved in the regulation of the nitrergic system in the supraoptic (SON) and paraventricular (PVN) nuclei under basal conditions. In this study we have assessed whether ERs are also involved in the modulation of the nitrergic system in the SON and PVN under acute systemic hypertonic conditions. Adult ovariectomized rats received a single injection of either estradiol, a selective ERα agonist, a selective ERβ agonist, a selective ERα antagonist, a selective ERβ antagonist or vehicle. Twenty-four hours later, animals received one i.p. injection of 1.5M NaCl to induce osmotic stress and were sacrificed after two additional hours. The number of NADPH-diaphorase-positive cells in the SON and PVN was determined. Their number in the SON was not affected by NaCl administration, whereas in the four PVN subdivisions it was decreased after NaCl administration. Estradiol and the ERα agonist prevented the action of NaCl in the four subdivisions of the PVN. In contrast, the inhibition of ERα enhanced the effect of NaCl, inducing a further decrease in the number of NADPH-diaphorase-positive cells. Moreover, the ERβ agonist enhanced and the ERβ antagonist blocked the effect of NaCl on the number of NADPH-diaphorase-positive neurons in the SON and in the medial magnocellular subdivision of the PVN. These findings indicate that estradiol regulates the nitrergic system in the SON and PVN under acute osmotic stress conditions, but the effects specifically depend on the anatomical subregions and different ERs.
在基础条件下，雌激素受体（ERs）α和β参与视上核（SON）和室旁核（PVN）中一氧化氮能系统的调节。在本研究中，我们评估了在急性全身性高渗条件下，ERs是否也参与SON和PVN中一氧化氮能系统的调节。成年去卵巢大鼠单次注射雌二醇、选择性ERα激动剂、选择性ERβ激动剂、选择性ERα拮抗剂、选择性ERβ拮抗剂或溶剂。24小时后，动物腹腔注射1.5M NaCl以诱导渗透压应激，并在另外两小时后处死。测定SON和PVN中NADPH-黄递酶阳性细胞的数量。SON中其数量不受NaCl给药的影响，而在PVN的四个亚区中，NaCl给药后数量减少。雌二醇和ERα激动剂可防止NaCl对PVN四个亚区的作用。相反，抑制ERα会增强NaCl的作用，导致NADPH-黄递酶阳性细胞数量进一步减少。此外，ERβ激动剂增强了NaCl对SON和PVN内侧大细胞亚区中NADPH-黄递酶阳性神经元数量的影响，而ERβ拮抗剂则阻断了这种影响。这些发现表明，在急性渗透压应激条件下，雌二醇调节SON和PVN中的一氧化氮能系统，但具体作用取决于解剖亚区和不同的ERs。
