实时监测血管平滑肌细胞内cGMP的时空动态变化。
Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells.
作者信息
Held Kara F, Dostmann Wolfgang R
机构信息
Department of Pharmacology, Yale University, New Haven, USA.
出版信息
Methods Mol Biol. 2013;1020:131-45. doi: 10.1007/978-1-62703-459-3_8.
Abstract
Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while -preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth muscle cells.
摘要
在保留哺乳动物细胞体内表型的同时，对其细胞内第二信使进行实时无创成像，需要结构精巧的生物传感器。在此，我们提供了在主动脉血管平滑肌细胞中利用单波长cGMP生物传感器δ-FlincG的方法。
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