Bauer J, Sokol L, Stríbrná J, Kremen M, Krajsová I, Hausner P, Hejnar P
Department of Oncology, Faculty of Medicine, Charles University, Prague, Czechoslovakia.
Neoplasma. 1990;37(3):233-8.
Tissue specimens from two melanoma patients and two patients with laryngeal carcinoma were studied for the expression of c-myc, N-myc and v-src oncogenes. Out of three different probes, only the N-myc probe one signalled amplification. While the two patients with laryngeal carcinoma showed only single copy of the N-myc gene in tumor cells, amplification of this gene was found in both two melanoma patients. The patients with 1 extra copy of the N-myc gene and its protein product had an early recurrence but is still alive, while the other melanoma patient with 4 extra copies of the gene, relapsed very early and died of melanoma within 7 months after diagnosis. Thus a higher amplification (4 extra copies) seems to correlate with very poor outcome of the disease.
对两名黑色素瘤患者和两名喉癌患者的组织标本进行了c-myc、N-myc和v-src癌基因表达的研究。在三种不同的探针中，只有N-myc探针1显示出扩增信号。两名喉癌患者的肿瘤细胞中仅显示N-myc基因的单拷贝，而两名黑色素瘤患者均发现该基因扩增。有1个额外N-myc基因拷贝及其蛋白质产物的患者出现早期复发，但仍存活，而另一名有4个额外基因拷贝的黑色素瘤患者复发很早，诊断后7个月内死于黑色素瘤。因此，更高的扩增倍数（4个额外拷贝）似乎与疾病的非常差的预后相关。
