Saksela K, Bergh J, Nilsson K
J Cell Biochem. 1986;31(4):297-304. doi: 10.1002/jcb.240310407.
c-myc oncogene is the most extensively studied member of the myc gene family, which now consists of three characterized members, namely the c-myc, N-myc, and L-myc genes. Deregulation owing to amplification and/or rearrangements of the c-myc gene have been described in a variety of human malignancies. Several neuroblastomas have amplifications of the N-myc genes. The c-myc, N-myc, or L-myc oncogenes are also found amplified in different cell lines from small cell carcinomas of the lung. In this study, we have examined the c-myc, N-myc, and c-erbB oncogenes in 34 clinical and autopsy tumor specimens representing various histopathological types of human lung cancer, including nine small cell lung cancers. A 30-fold amplification of the N-myc gene was found in a tumor histopathologically and histochemically verified as a typical adenocarcinoma. No amplifications of the c-myc or c-erbB oncogenes were seen in any of the tumors. In the DNA of one small cell carcinoma, an extra c-myc and N-myc cross-hybridizing restriction fragment was observed, possibly owing to an amplification of a yet uncharacterized myc-related gene.
c-myc癌基因是myc基因家族中研究最为广泛的成员，该家族目前由三个已明确的成员组成，即c-myc、N-myc和L-myc基因。在多种人类恶性肿瘤中均有因c-myc基因扩增和/或重排导致的失调现象。一些神经母细胞瘤存在N-myc基因的扩增。在来自肺小细胞癌的不同细胞系中也发现c-myc、N-myc或L-myc癌基因发生扩增。在本研究中，我们检测了34份临床和尸检肿瘤标本中的c-myc、N-myc和c-erbB癌基因，这些标本代表了各种组织病理学类型的人类肺癌，其中包括9例小细胞肺癌。在一例经组织病理学和组织化学证实为典型腺癌的肿瘤中发现N-myc基因有30倍的扩增。在任何肿瘤中均未观察到c-myc或c-erbB癌基因的扩增。在一例小细胞癌的DNA中，观察到一个额外的c-myc和N-myc交叉杂交限制片段，这可能是由于一个尚未明确的与myc相关基因的扩增所致。
