Rodríguez-Arellanes Gabriela, Nascimento de Sousa Carolina, de Medeiros-Muniz Mauro, Ramírez José A, Pizzini Cláudia V, de Abreu-Almeida Marcos, Evangelista de Oliveira Manoel M, Fusco-Almeida Ana-Marisa, Vite-Garín Tania, Pitangui Nayla S, Estrada-Bárcenas Daniel A, González-González Antonio E, Mendes-Giannini María José Soares, Zancopé-Oliveira Rosely M, Taylor Maria-Lucia
Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, Mexico.
Eukaryot Cell. 2013 Jul;12(7):1033-8. doi: 10.1128/EC.00012-13. Epub 2013 May 24.
The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+ mating type) and G-186AR (- mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective + and - mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources.
通过聚合酶链反应（PCR）鉴定了与荚膜组织胞浆菌MAT1位点相关的MAT1-1和MAT1-2等位基因。共研究了28株真菌分离株，其中6株来自人类临床样本，22株来自环境样本（感染蝙蝠和受污染土壤）。在来自墨西哥的14株分离株中，71.4%（95%置信区间[95%CI]，48.3%至94.5%）为MAT1-2基因型，而来自巴西的所有分离株均为MAT1-1基因型。以G-217B（+交配型）和G-186AR（-交配型）菌株的序列为参考，对每个MAT1等位基因区域进行测序和比对。对所研究的MAT1-1和MAT1-2序列进行的BLASTn分析与其各自的+和-交配型基因型相关。通过最大似然（ML）法生成树状图，以寻找每个MAT1等位基因分离株之间的相似性。所有源自巴西蝙蝠的MAT1-1分离株形成一个明确的组；来自墨西哥的3株分离株、G-217B菌株以及一个包含所有土壤来源分离株和来自巴西的2株临床分离株的亚组形成第二组；最后，在墨西哥捕获的一只迁徙蝙蝠的1株分离株（EH-696P）形成MAT1-1基因型的第三组。MAT1-2等位基因形成两组，其中一组包括来自感染蝙蝠的2株荚膜组织胞浆菌分离株，它们与G-186AR菌株密切相关。另一组由2株人类分离株和6株来自感染蝙蝠的分离株组成。带有内部转录间隔区1（ITS1）-5.8S-ITS2和MAT1-1或MAT1-2序列的串联ML树支持MAT1-1或MAT1-2分离株之间的相关性。荚膜组织胞浆菌的交配型与分离株的地理来源相关，并且来自巴西的所有分离株与其环境来源相关。
