Kasperczyk Sławomir, Słowińska-Łożyńska Ludmiła, Kasperczyk Aleksandra, Wielkoszyński Tomasz, Birkner Ewa
Department of Biochemistry, Medical University of Silesia in Katowice, Jordana, Zabrze, Poland.
Department of Biophysics, Medical University of Silesia, Jordana, Zabrze, Poland.
Toxicol Ind Health. 2015 Dec;31(12):1165-71. doi: 10.1177/0748233713491804. Epub 2013 May 24.
The aim of the study was to investigate the influence of occupational lead (Pb) exposure on lipid peroxidation, protein carbonylation, and plasma viscosity in workers. The examined group included 283 healthy male employees of manufacturing facilities using zinc and Pb. The mean blood concentrations of Pb and zinc protoporphyrin as well as the mean urine δ-aminolevulinic acid levels were used as markers of exposure for the examined group. Taking into account the obtained mean values of blood lead level, the examined group was divided into three subgroups. When comparing the control group with the subgroups, Pb exposure markers were significantly elevated in all the three subgroups. Concentrations of conjugated dienes (CD), lipid hydroperoxides, malondialdehyde (MDA), and protein carbonyl groups were also significantly increased. Conversely, the levels of total protein and protein sulfhydryls were significantly decreased in the subgroups compared with the controls. The plasma viscosity was significantly elevated in the subgroups. A dose-response between Pb levels and plasma viscosity was not observed. Pb supposedly elevates MDA and CD in a dose-dependent manner. In conclusion, occupational Pb exposure induces oxidative stress that results in lipid and protein damage. Moreover, Pb-induced oxidative stress is likely the primary factor that elevates plasma viscosity, despite decreased protein levels.
本研究的目的是调查职业性铅（Pb）暴露对工人脂质过氧化、蛋白质羰基化和血浆粘度的影响。受检组包括283名使用锌和铅的制造工厂的健康男性员工。铅和锌原卟啉的平均血浓度以及尿δ-氨基乙酰丙酸的平均水平被用作受检组的暴露标志物。根据所获得的血铅水平均值，将受检组分为三个亚组。当将对照组与各亚组进行比较时，所有三个亚组的铅暴露标志物均显著升高。共轭二烯（CD）、脂质氢过氧化物、丙二醛（MDA）和蛋白质羰基的浓度也显著增加。相反，与对照组相比，各亚组的总蛋白和蛋白质巯基水平显著降低。各亚组的血浆粘度显著升高。未观察到铅水平与血浆粘度之间的剂量反应关系。铅可能以剂量依赖的方式升高MDA和CD。总之，职业性铅暴露会引发氧化应激，导致脂质和蛋白质损伤。此外，尽管蛋白质水平降低，但铅诱导的氧化应激可能是血浆粘度升高的主要因素。
