Laboratory of Zoology, Regional University of Cariri-URCA, Pimenta, 63105-000 Crato, CE, Brazil.
Evid Based Complement Alternat Med. 2013;2013:913671. doi: 10.1155/2013/913671. Epub 2013 Apr 23.
Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the "Caatinga" biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the body fats of L. macrosternum and L. vastus and to evaluate their antimicrobial activities as well as the ecological implications of their use in traditional folk medicine. Oils were extracted from body fat located in the ventral region of L. macrosternum (OLM) and L. vastus (OLV) using hexane as a solvent. The fatty acids were identified by GC-MS. The antimicrobial activities of the oils, either alone or in combination with antibiotics and antifungal drugs, were tested on standard strains of microorganisms as well as on multiresistant strains of Escherichia coli and Staphylococcus. OLM contained 40% saturated and 60% unsaturated fatty acids, while OLV contained 58.33% saturated and 41.67% unsaturated fatty acids. Our results indicated that both OLM and OLV demonstrated relevant antimicrobial activities (with MIC 256 μ g/mL for both) against Pseudomonas aeruginosa and Candida krusei. However, no antimicrobial effects were observed when these oils were combined with antibiotics or antifungal drugs.
大蟾蜍属（Leptodactylidae 科）中的大蟾蜍（Leptodactylus macrosternum）和蟾属（L. vastus）在巴西北部的“Caatinga”生物群中很常见。蟾属的身体脂肪被用作治疗多种人类疾病的动物治疗剂。本工作旨在确定大蟾蜍属和蟾属身体脂肪的化学组成，并评估其抗菌活性以及在传统民间医学中使用的生态意义。使用正己烷作为溶剂，从大蟾蜍属（OLM）和蟾属（OLV）腹部的体脂中提取油。通过 GC-MS 鉴定脂肪酸。单独或与抗生素和抗真菌药物联合使用这些油，对标准微生物菌株以及多药耐药性大肠杆菌和金黄色葡萄球菌菌株进行了抗菌活性测试。OLM 含有 40%的饱和脂肪酸和 60%的不饱和脂肪酸，而 OLV 含有 58.33%的饱和脂肪酸和 41.67%的不饱和脂肪酸。我们的结果表明，OLM 和 OLV 均对铜绿假单胞菌和克柔假丝酵母表现出相关的抗菌活性（MIC256μ g/mL）。然而，当这些油与抗生素或抗真菌药物联合使用时，并未观察到抗菌作用。
