Chen Wei, Liu Zhong-juan, Ye Jing
Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Feb;48(2):128-34.
To explore the relationship among the expression of GSK3β, PI3K/Akt signaling transduction pathway,and cytokines IL6 in Chronic Rhinosinusitis.
We assayed mRNA and protein for GSK3β, PI3K, Akt by using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), and measured the cytokines IL6 mRNA by using reverse transcription polymerase chain reaction (RT-PCR) in the nasal tissue from the patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP), Chronic Rhinosinusitis without Nasal Polyps (CRSsNP) and control subjects.
The relative expression levels of GSK3β, PI3K, Akt and IL-6 in CRSwNP were 0.6254 ± 0.0584, 0.8239 ± 0.7186, 0.9369 ± 0.0823 and 0.8973 ± 0.0680. But the relative expression levels of GSK3β, PI3K, Akt and IL-6 in control subjects were 0.2684 ± 0.0726, 0.3578 ± 0.0994, 0.6721 ± 0.0590, 0.5898 ± 0.0891. There were significant differences between the groups of CRSwNP and control subjects (t values were 2.358, 3.071, 2.764, and 2.239, respectively, all P < 0.05). There was no significant difference of GSK3β, PI3K, Akt and GSK3β between the groups of CRSwNP and CRSsNP (t values were 1.597, 1.842, 1.468 and 0.926, respectively, all P < 0.05). GSK3β, PI3K, Akt were not only expressed in the cytoplasm of the epithelium and gland cells, but also in the cytoplasm of the inflammatory cell. GSK3β, PI3K, Akt protein in CRS were detected at a higher rate than the normal nasal tissue (values were 16.42, 16.25 and 15.57,respectively, all P < 0.01). However there was no significant difference of GSK3β, PI3K, Akt protein between the groups of CRSwNP and CRSsNP (values were 3.27, 2.85 and 2.46, respectively, all P > 0.05). There was a positive correlation trend among the expression of GSK3β, PI3K and Akt, and IL-6 in CRS (r values were 0.645, 0.617 and 0.583,respectively, all P < 0.01).
The abnormal expression of IL-6, PI3K, Akt and GSK3β in the nasal mucosa of CRS may play a pro-inflammatory role in the occurrence and development of CRS.
探讨慢性鼻-鼻窦炎中糖原合成酶激酶3β（GSK3β）、磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）信号转导通路的表达与细胞因子白细胞介素6（IL-6）之间的关系。
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学（IHC）法检测慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）、不伴鼻息肉的慢性鼻-鼻窦炎（CRSsNP）患者及对照者鼻黏膜组织中GSK3β、PI3K、Akt的mRNA和蛋白表达，并用RT-PCR法检测IL-6 mRNA水平。
CRSwNP组中GSK3β、PI3K、Akt及IL-6的相对表达水平分别为0.6254±0.0584、0.8239±0.7186、0.9369±0.0823和0.8973±0.0680。而对照组中GSK3β、PI3K、Akt及IL-6的相对表达水平分别为0.2684±0.0726、0.3578±0.0994、0.6721±0.0590和0.5898±0.0891。CRSwNP组与对照组之间差异有统计学意义（t值分别为2.358、3.071、2.764和2.239，均P<0.05）。CRSwNP组与CRSsNP组之间GSK3β、PI3K、Akt及IL-6差异无统计学意义（t值分别为1.597、1.842、1.468和0.926，均P>0.05）。GSK3β、PI3K、Akt不仅表达于上皮细胞和腺细胞的胞质中，也表达于炎性细胞的胞质中。CRS患者鼻黏膜组织中GSK3β、PI3K、Akt蛋白的检出率高于正常鼻黏膜组织（分别为16.42、16.25和15.57，均P<0.01）。然而，CRSwNP组与CRSsNP组之间GSK3β、PI3K、Akt蛋白差异无统计学意义（分别为3.27、2.85和2.46，均P>0.05）。CRS中GSK3β、PI3K、Akt与IL-6的表达呈正相关趋势（r值分别为0.645、0.617和0.583，均P<0.01）。
CRS鼻黏膜中IL-6、PI3K、Akt及GSK3β的异常表达可能在CRS的发生发展中起促炎作用。
