Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, Seoul, South Korea.
J Allergy Clin Immunol. 2013 Mar;131(3):772-80. doi: 10.1016/j.jaci.2012.12.671. Epub 2013 Jan 30.
Chronic rhinosinusitis with nasal polyps (CRSwNP) or without nasal polyps (CRSsNP) is associated with expression of various cytokines. Suppressors of cytokine signaling (SOCS) regulate cytokine activity in a variety of cells, modulating inflammatory responses.
We analyzed the expression and distribution pattern of SOCS1 and SOCS3 in CRSwNP and CRSsNP, and their cytokine-driven expression regulation in sinus mucosa. In addition, the expression levels of various cytokines were evaluated in CRSwNP and CRSsNP.
The expression levels of SOCS1 and SOCS3 in CRSwNP and CRSsNP and in control samples were assessed by using real-time PCR, Western blot, and immunohistochemistry. Nasal epithelial cell culture was used to elucidate the effect of IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, TNF-α, and TGF-β1 on SOCS1 and SOCS3 expression in sinus mucosa. The expression levels of these cytokines were also evaluated in normal and inflammatory sinus mucosa by using real-time PCR and Western blot.
The expression levels of SOCS1 and SOCS3 were increased in CRS, irrespective of the presence of nasal polyp, and they were distributed in superficial epithelium, submucosal glands, and vascular endothelium in sinus mucosa. SOCS1 was induced by IL-4, IL-13, IFN-γ, and TNF-α, while SOCS3 expression was upregulated by IL-6, IL-13, IFN-γ, and TNF-α. IL-4 and IL-13 levels were increased in CRSwNP, while IL-4, IFN-γ, TNF-α, and TGF-β1 levels were increased in CRSsNP.
SOCS1 and SOCS3 are increased in CRS, irrespective of nasal polyp presence. This may be a response to elevated levels of various cytokines increasingly expressed in inflammatory sinus mucosa.
伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）或不伴鼻息肉的慢性鼻-鼻窦炎（CRSsNP）与各种细胞因子的表达有关。细胞因子信号转导抑制因子（SOCS）在多种细胞中调节细胞因子的活性，调节炎症反应。
我们分析了 SOCS1 和 SOCS3 在 CRSwNP 和 CRSsNP 中的表达和分布模式，以及它们在鼻窦黏膜中细胞因子驱动的表达调控。此外，还评估了 CRSwNP 和 CRSsNP 中各种细胞因子的表达水平。
使用实时 PCR、Western blot 和免疫组织化学分析 CRSwNP 和 CRSsNP 以及对照样本中 SOCS1 和 SOCS3 的表达水平。鼻上皮细胞培养用于阐明 IL-4、IL-5、IL-6、IL-10、IL-13、IFN-γ、TNF-α 和 TGF-β1 对鼻窦黏膜中 SOCS1 和 SOCS3 表达的影响。还通过实时 PCR 和 Western blot 评估了这些细胞因子在正常和炎症性鼻窦黏膜中的表达水平。
SOCS1 和 SOCS3 的表达水平在 CRS 中均升高，无论是否存在鼻息肉，并且它们在鼻窦黏膜的浅表上皮、黏膜下腺和血管内皮中分布。SOCS1 由 IL-4、IL-13、IFN-γ 和 TNF-α 诱导，而 SOCS3 表达受 IL-6、IL-13、IFN-γ 和 TNF-α 上调。CRSwNP 中 IL-4 和 IL-13 水平升高，而 CRSsNP 中 IL-4、IFN-γ、TNF-α 和 TGF-β1 水平升高。
SOCS1 和 SOCS3 在 CRS 中均升高，无论是否存在鼻息肉。这可能是对炎症性鼻窦黏膜中日益表达的各种细胞因子水平升高的一种反应。
