Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Sep;113:107-14. doi: 10.1016/j.saa.2013.04.064. Epub 2013 May 3.
Four simple, specific, accurate and precise spectrophotometric methods manipulating ratio spectra were developed and validated for simultaneous determination of simvastatin (SM) and ezetimibe (EZ) namely; extended ratio subtraction (EXRSM), simultaneous ratio subtraction (SRSM), ratio difference (RDSM) and absorption factor (AFM). The proposed spectrophotometric procedures do not require any preliminary separation step. The accuracy, precision and linearity ranges of the proposed methods were determined, and the methods were validated and the specificity was assessed by analyzing synthetic mixtures containing the cited drugs. The four methods were applied for the determination of the cited drugs in tablets and the obtained results were statistically compared with each other and with those of a reported HPLC method. The comparison showed that there is no significant difference between the proposed methods and the reported method regarding both accuracy and precision.
四种简单、特定、准确和精密的分光光度法通过比值光谱处理被开发并验证，可用于同时测定辛伐他汀（SM）和依折麦布（EZ），分别为：扩展比值扣除法（EXRSM）、同时比值扣除法（SRSM）、比值差法（RDSM）和吸收因子法（AFM）。所提出的分光光度法不需要任何预先的分离步骤。通过分析含有被检测药物的合成混合物，确定了所提出方法的准确性、精密度和线性范围，并对方法进行了验证，评估了特异性。四种方法均应用于片剂中被检测药物的测定，所得结果与文献报道的 HPLC 方法进行了统计学比较。比较表明，在所研究的方法中，无论是准确性还是精密度，与文献报道的方法之间均无显著差异。
