一种用于快速同时检测人血样本中布鲁氏菌属的双重 PCR 方法。

A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.

机构信息

Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

出版信息

Asian Pac J Trop Med. 2013 Jun;6(6):453-6. doi: 10.1016/S1995-7645(13)60073-5.

DOI:10.1016/S1995-7645(13)60073-5

PMID:23711705

Abstract

OBJECTIVE

To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples.

METHODS

Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.

RESULTS

Of the 52 peripheral bloods samples tested, 25 sample (48%) showed positive reactions in PCR. Twelve samples were positive for Brucella abortus 39 (B. abortus 39) (23%), 13 for Brucella melitensis 39 (B. melitensis 39) (25%) and 0 for Brucella ovis 39 (B. ovis 39) (0%).

CONCLUSIONS

This work demonstrates that in case where specific primers were utilized, duplex PCR has proved to be a simple, fast, and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.

摘要

目的

设计一种双重 PCR 方法，用于快速同时检测人血样本中的布鲁氏菌属物种。

方法

从疑似布鲁氏菌病患者中采集了 52 份外周血样本。提取 DNA 后，使用三对引物进行 PCR 检测，这些引物可以同时鉴定和区分人类和动物中三种主要的致病性布鲁氏菌。

结果

在检测的 52 份外周血样本中，25 份（48%）PCR 呈阳性反应。12 份为流产布鲁氏菌 39 型（B. abortus 39）（23%）阳性，13 份为马耳他布鲁氏菌 39 型（B. melitensis 39）（25%）阳性，0 份为绵羊布鲁氏菌 39 型（B. ovis 39）阳性（0%）。