Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Asian Pac J Trop Med. 2013 Jun;6(6):453-6. doi: 10.1016/S1995-7645(13)60073-5.
To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples.
Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.
Of the 52 peripheral bloods samples tested, 25 sample (48%) showed positive reactions in PCR. Twelve samples were positive for Brucella abortus 39 (B. abortus 39) (23%), 13 for Brucella melitensis 39 (B. melitensis 39) (25%) and 0 for Brucella ovis 39 (B. ovis 39) (0%).
This work demonstrates that in case where specific primers were utilized, duplex PCR has proved to be a simple, fast, and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.
设计一种双重 PCR 方法，用于快速同时检测人血样本中的布鲁氏菌属物种。
从疑似布鲁氏菌病患者中采集了 52 份外周血样本。提取 DNA 后，使用三对引物进行 PCR 检测，这些引物可以同时鉴定和区分人类和动物中三种主要的致病性布鲁氏菌。
在检测的 52 份外周血样本中，25 份（48%）PCR 呈阳性反应。12 份为流产布鲁氏菌 39 型（B. abortus 39）（23%）阳性，13 份为马耳他布鲁氏菌 39 型（B. melitensis 39）（25%）阳性，0 份为绵羊布鲁氏菌 39 型（B. ovis 39）阳性（0%）。
本研究表明，在使用特定引物的情况下，双重 PCR 已被证明是一种简单、快速且相对廉价的方法，可用于同时检测临床样本中的重要布鲁氏菌属物种。
