全反式维甲酸诱导急性早幼粒细胞白血病细胞中转录因子 CEBPB 的靶基因鉴定。
Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid.
机构信息
Department of Hepatic Surgery, National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Central South University, China.
出版信息
Asian Pac J Trop Med. 2013 Jun;6(6):473-80. doi: 10.1016/S1995-7645(13)60077-2.
OBJECTIVE
To identify target genes of transcription factor CCAAT enhancer-binding protein β (CEBPB) in acute promyelocytic leukemia cells induced by all-trans retinoic acid.
METHODS
A new strategy for high-throughput identification of direct target genes was established by combining chromatin immunoprecipitation (ChIP) with in vitro selection. Then, 106 potential CEBPB binding fragments from the genome of the all-trans retinoic acid (ATRA)-treated NB4 cells were identified.
RESULTS
Of them, 82 were mapped in proximity to known or previously predicted genes; 7 were randomly picked up for further confirmation by ChIP-PCR and 3 genes (GALM, ITPR2 and ORM2) were found to be specifically up-regulated in the ATRA-treated NB4 cells, indicating that they might be the down-stream target genes of ATRA.
CONCLUSIONS
Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.
目的
鉴定全反式维甲酸诱导的急性早幼粒细胞白血病细胞中转录因子 CCAAT 增强子结合蛋白 β（CEBPB）的靶基因。
方法
通过将染色质免疫沉淀（ChIP）与体外选择相结合，建立了一种高通量鉴定直接靶基因的新策略。然后，从全反式维甲酸（ATRA）处理的 NB4 细胞的基因组中鉴定出 106 个潜在的 CEBPB 结合片段。
结果
其中，82 个与已知或先前预测的基因接近；随机挑选 7 个进行 ChIP-PCR 进一步验证，发现 3 个基因（GALM、ITPR2 和 ORM2）在 ATRA 处理的 NB4 细胞中特异性上调，表明它们可能是 ATRA 的下游靶基因。
结论
我们的研究结果为 ATRA 诱导的粒细胞分化机制提供了新的见解。
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