Molecular Inflammation Research Center for Aging Intervention and College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
J Ethnopharmacol. 2013 Jul 30;148(3):890-4. doi: 10.1016/j.jep.2013.05.037. Epub 2013 May 25.
To elucidate the anti-allergic and anti-inflammatory effects of Petasites genus, we studied the effects of several compounds isolated from Petasites japonicas leaves.
Bakkenolide B was isolated from Petasites japonicus leaves. Antigen-induced degranulation was measured in RBL-2H3 mast cells by measuring β-hexosamidase activity. Induction of inducible nitric oxide synthase and cyclooxygenase 2 was measured by Western blotting in peritoneal macrophages. Ovalbumin-induced asthma model was used for in vivo efficacy test of bakkanolide B.
We found that bakkenolide B, a major component of the leaves, concentration-dependently inhibited RBL-2H3 mast cell degranulation. Bakkenolide B also inhibited the gene inductions of inducible nitric oxide synthase and cyclooxygenase 2 in mouse peritoneal macrophages. Furthermore, in an ovalbumin-induced asthma model, bakkenolide B strongly inhibited the accumulation of eosinophils, macrophages, and lymphocytes to bronchoalveolar lavage fluid.
Bakkenolide B has suppressive properties for allergic and inflammatory responses and may be utilized as a potent agent for the treatment of asthma.
为阐明山金车属的抗过敏和抗炎作用，我们研究了从山金车属植物叶子中分离得到的几种化合物的作用。
从山金车属植物叶子中分离出 Bakkenolide B。通过测量β-己糖胺酶活性来测量 RBL-2H3 肥大细胞中的抗原诱导脱粒。通过 Western blot 测量诱导型一氧化氮合酶和环氧化酶 2 的诱导在腹腔巨噬细胞中。使用卵清蛋白诱导的哮喘模型进行 Bakkenolide B 的体内功效测试。
我们发现，山金车属植物叶子中的主要成分 Bakkenolide B 浓度依赖性地抑制 RBL-2H3 肥大细胞脱粒。Bakkenolide B 还抑制了小鼠腹腔巨噬细胞中诱导型一氧化氮合酶和环氧化酶 2 的基因诱导。此外，在卵清蛋白诱导的哮喘模型中，Bakkenolide B 强烈抑制嗜酸性粒细胞、巨噬细胞和淋巴细胞向支气管肺泡灌洗液的积聚。
Bakkenolide B 对过敏和炎症反应具有抑制作用，可作为治疗哮喘的有效药物。
