Unité de Recherche des Substances Actives Marines (URSAM), Laboratoire de Pharmacologie, Faculté de pharmacie, Université de Monastir, Tunisia.
Unité de Recherche des Substances Actives Marines (URSAM), Laboratoire de Pharmacologie, Faculté de pharmacie, Université de Monastir, Tunisia.
Environ Toxicol Pharmacol. 2013 Sep;36(2):339-346. doi: 10.1016/j.etap.2013.04.014. Epub 2013 May 6.
The present study was conducted to evaluate the antiproliferative and antioxidant activities of organic extract and its polar fractions from Eunicella singularis (Esper 1794). Organic extract and two fractions of E. singularis (F2 and F3) were screened for the presence of phenolic compounds, terpenoids and glycosides. The antiproliferative activity of E. singularis organic extract and its polar fractions was evaluated on human cancer cell lines (A549, lung cell carcinoma; HCT15, colon cell carcinoma and MCF7, breast adenocarcinoma), using the MTT colorimetric method and clonogenic assay, as well as the antioxidant activity, using the stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the FRAP assays. The fractions F2 and F3 showed significant total phenolic content (40 and 35.72mg gallic-acid equivalent/g dried sample), respectively, and important antiproliferative properties against the cancer cell lines. The IC50 values, ranged from 36 to 274μg/ml for A549; 93 to 426μg/ml for HCT15; and 52 to 225μg/ml for MCF7 and in the clonogenic inhibition assay from 18 to 134μg/ml for A549; 43 to 357μg/ml for HCT15; and 17 to 160μg/ml for MCF7. Using the DPPH method, the fraction F2 exhibited the strongest radical scavenging activity, with IC50 0.08mg/ml, which approaches the activity of the powerful antioxidant standard, ascorbic acid (IC50=0.064mg/ml). The reducing power of the samples was in the following order: F2>organic extract>F3. These results suggest that E. singularis fractions might be used as a potential source of natural antioxidant and antitumor agents. The purification and determination of the chemical structures of compounds in these active fractions are under investigation. The results could provide a compound(s) with a promising role in future medicines.
本研究旨在评估 Eunicella singularis（Esper 1794）的有机提取物及其极性部分的抗增殖和抗氧化活性。对 E. singularis 的有机提取物和两个极性部分（F2 和 F3）进行了筛选，以检测其是否存在酚类化合物、萜类化合物和糖苷。采用 MTT 比色法和集落形成实验评价 E. singularis 有机提取物及其极性部分对人癌细胞系（A549，肺癌；HCT15，结肠癌细胞和 MCF7，乳腺癌）的抗增殖活性，以及使用稳定自由基 1,1-二苯基-2-苦基肼（DPPH）和 FRAP 测定法评估抗氧化活性。F2 和 F3 部分分别显示出显著的总酚含量（40 和 35.72mg 没食子酸当量/g 干样品），并对癌细胞系表现出重要的抗增殖作用。IC50 值范围为 A549 为 36 至 274μg/ml；HCT15 为 93 至 426μg/ml；MCF7 为 52 至 225μg/ml；在集落抑制实验中，A549 为 18 至 134μg/ml；HCT15 为 43 至 357μg/ml；MCF7 为 17 至 160μg/ml。使用 DPPH 法，F2 部分表现出最强的自由基清除活性，IC50 为 0.08mg/ml，接近强大抗氧化剂标准抗坏血酸（IC50=0.064mg/ml）的活性。样品的还原能力依次为：F2>有机提取物>F3。这些结果表明，E. singularis 部分可能可作为天然抗氧化和抗肿瘤药物的潜在来源。目前正在研究这些活性部分中化合物的分离和化学结构的确定。研究结果可能为未来药物提供具有潜在作用的化合物。
