Kumar Amit, Peterson Greg, Nagaraja Tiruvoor G, Narayanan Sanjeev
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.
J Basic Microbiol. 2014 Aug;54(8):812-7. doi: 10.1002/jobm.201200748. Epub 2013 May 26.
Fusobacterium necrophorum, classified into subsp. necrophorum (Fnn) and subsp. funduliforme (Fnf), is frequently associated with necrotic infections of animals and humans. The outer membrane proteins (OMP) of many Gram negative bacteria play an important role in bacterial adhesion and establishment of infection. The OMP profile of F. necrophorum has not been well characterized. We analyzed OMP of bovine strains of Fnn and Fnf and human strains of F. necrophorum. Electrophoretic separations of extracted OMP of Fnn and Fnf strains of cattle showed a total of 19 and 20 protein bands, respectively. The most prominent protein band was 40 kDa in Fnn and 37.5 kDa in Fnf. The four human clinical strains examined had more heterogeneous banding patterns and had different profiles than those of bovine Fnf strains. A total of 11 protein bands in Fnn and 13 protein bands in Fnf were recognized by sera from cattle with liver abscesses. The intensities of many of the bands in Fnn were higher than that of Fnf. We conclude that the two subspecies of F. necrophorum differ in their OMP profiles and the difference may account for differences in their virulence and involvement in the pathogenesis of necrotic infections.
坏死梭杆菌分为坏死亚种（Fnn）和具沟亚种（Fnf），常与动物和人类的坏死性感染有关。许多革兰氏阴性菌的外膜蛋白（OMP）在细菌黏附和感染的建立中起重要作用。坏死梭杆菌的OMP谱尚未得到很好的表征。我们分析了Fnn和Fnf的牛源菌株以及坏死梭杆菌的人源菌株的OMP。对牛的Fnn和Fnf菌株提取的OMP进行电泳分离，分别显示出总共19条和20条蛋白带。最突出的蛋白带在Fnn中为40 kDa，在Fnf中为37.5 kDa。所检测的4株人临床菌株具有更异质的条带模式，且与牛的Fnf菌株的条带模式不同。肝脓肿牛的血清识别出Fnn中的总共11条蛋白带和Fnf中的13条蛋白带。Fnn中许多条带的强度高于Fnf。我们得出结论，坏死梭杆菌的两个亚种在OMP谱上存在差异，这种差异可能解释了它们在毒力和坏死性感染发病机制中的差异。
