Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, People's Republic of China.
Cell Biochem Biophys. 2014 Jan;68(1):159-62. doi: 10.1007/s12013-013-9682-9.
This study aims to compare the different latent infections of herpes simplex virus type I in a murine model. One hundred and twenty BALB/c mice were randomly assigned into either of three groups: intravenous inoculation group, ocular abrasion group, and intranasal inoculation group. Six weeks later, the trigeminal ganglia (TG) were removed to detect the expression of HSV-I antigen. HSV DNA in TG was also detected by polymerase chain reaction to confirm latent infection. The rate of HSV DNA in TG detected in the intravenous inoculation group was 18/22 and 22/26 in the ocular abrasion group, both of which were higher than the rate detected in the intranasal inoculation group (18/30). The expression of HSV antigen in TG in these three groups was all negative. Mortality rate in the intravenous inoculation group was 8/30, which was much higher than those of the two other groups. Intranasal virus dripping, cornea abrasion, and intravenous injection can detect latent HSV-I infection in a murine model. Compared to two other groups, the cornea abrasion group showed less severe signs, a quicker recovery rate in acute infection, and higher incidence rate of latent infection. Therefore, it is an ideal method in the presence of latent HSV-I infection.
本研究旨在比较单纯疱疹病毒 I 型在小鼠模型中的不同潜伏感染。将 120 只 BALB/c 小鼠随机分为三组：静脉接种组、眼擦伤组和鼻腔接种组。6 周后，取出三叉神经节（TG）检测 HSV-I 抗原的表达。通过聚合酶链反应检测 TG 中的 HSV DNA，以确认潜伏感染。静脉接种组 TG 中 HSV DNA 的检出率为 18/22，眼擦伤组为 22/26，均高于鼻腔接种组（18/30）。这三组 TG 中 HSV 抗原的表达均为阴性。静脉接种组的死亡率为 8/30，明显高于其他两组。鼻腔病毒滴注、角膜擦伤和静脉注射均可在小鼠模型中检测到潜伏的 HSV-I 感染。与另外两组相比，角膜擦伤组的症状较轻，急性感染的恢复速度较快，潜伏感染的发生率较高。因此，在存在潜伏 HSV-I 感染的情况下，它是一种理想的方法。
