Strassburg Sandra, Nienhueser Henrik, Björn Stark G, Finkenzeller Günter, Torio-Padron Nestor
Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
J Tissue Eng Regen Med. 2016 Jun;10(6):496-506. doi: 10.1002/term.1769. Epub 2013 May 27.
Neovascularization of adipose tissue equivalents is a crucial step in successful adipose tissue engineering, since insufficient vascularization results in graft resorption in an in vivo situation. A possible cellular approach to overcome this limitation is the co-implantation of adipose-derived stem cells (ASCs) with endothelial cells to stimulate the formation of a vascular network. We investigated the potential of ASCs derived from human abdominal fat tissue co-cultured with endothelial progenitor cells (EPCs) from human peripheral blood to stimulate neovascularization of fibrin constructs on the chorioallantoic membrane (CAM) of fertilized chicken eggs, in direct comparison to human umbilical vein endothelial cells (HUVECs). After 9 days of incubation, cell-fibrin constructs were explanted and histologically evaluated with respect to ingrowth of avian blood vessels into the construct and formation of human blood vessels by co-implanted endothelial cells. When administered on the CAM, ASCs successfully guided host vasculature into the construct (angiogenesis) and guided formation of capillary-like structures by co-implanted human endothelial cells (vasculogenesis), with HUVECs being superior to EPCs, leading to a perfused avian and human capillary network within the fibrin construct. However, the results also showed that perfused human blood vessels were only observed near the CAM compared to unperfused capillary-like structures near the top of the construct, indicating that perfusion of the cell-fibrin construct takes longer than 9 days. In conclusion, as blood vessel formation is an essential step during adipogenic differentiation, the data support our hypothesis that cellular communication between transplanted ASCs and endothelial cells is beneficial for vasculogenesis. Copyright © 2013 John Wiley & Sons, Ltd.
脂肪组织类似物的血管新生是成功进行脂肪组织工程的关键步骤，因为血管化不足会导致体内移植组织的吸收。一种可能克服这一限制的细胞方法是将脂肪来源的干细胞（ASC）与内皮细胞共同植入，以刺激血管网络的形成。我们研究了源自人腹部脂肪组织的ASC与源自人外周血的内皮祖细胞（EPC）共培养，以刺激受精鸡蛋绒毛尿囊膜（CAM）上纤维蛋白构建体血管新生的潜力，并与人类脐静脉内皮细胞（HUVEC）进行直接比较。孵育9天后，取出细胞 - 纤维蛋白构建体，并对禽类血管向构建体内生长以及共植入的内皮细胞形成人类血管的情况进行组织学评估。当在CAM上施用时，ASC成功引导宿主血管进入构建体（血管生成），并引导共植入的人类内皮细胞形成毛细血管样结构（血管发生），HUVEC比EPC更具优势，从而在纤维蛋白构建体内形成灌注的禽类和人类毛细血管网络。然而，结果还表明，与构建体顶部附近未灌注的毛细血管样结构相比，仅在CAM附近观察到灌注的人类血管，这表明细胞 - 纤维蛋白构建体的灌注时间超过9天。总之，由于血管形成是脂肪生成分化过程中的一个重要步骤，这些数据支持了我们的假设，即移植的ASC与内皮细胞之间的细胞通讯有利于血管发生。版权所有© 2013 John Wiley & Sons, Ltd.
