Department of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
Institute for Molecular and Cellular Anatomy, Faculty for Biology and Preclinical Medicine, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
Cells. 2023 Feb 12;12(4):592. doi: 10.3390/cells12040592.
With a history of more than 100 years of different applications in various scientific fields, the chicken chorioallantoic membrane (CAM) assay has proven itself to be an exceptional scientific model that meets the requirements of the replacement, reduction, and refinement principle (3R principle). As one of three extraembryonic avian membranes, the CAM is responsible for fetal respiration, metabolism, and protection. The model provides a unique constellation of immunological, vascular, and extracellular properties while being affordable and reliable at the same time. It can be utilized for research purposes in cancer biology, angiogenesis, virology, and toxicology and has recently been used for biochemistry, pharmaceutical research, and stem cell biology. Stem cells and, in particular, mesenchymal stem cells derived from adipose tissue (ADSCs) are emerging subjects for novel therapeutic strategies in the fields of tissue regeneration and personalized medicine. Because of their easy accessibility, differentiation profile, immunomodulatory properties, and cytokine repertoire, ADSCs have already been established for different preclinical applications in the files mentioned above. In this review, we aim to highlight and identify some of the cross-sections for the potential utilization of the CAM model for ADSC studies with a focus on wound healing and tissue engineering, as well as oncological research, e.g., sarcomas. Hereby, the focus lies on the combination of existing evidence and experience of such intersections with a potential utilization of the CAM model for further research on ADSCs.
鸡胚尿囊膜（CAM）检测法已有超过 100 年的历史，在各个科学领域都有不同的应用，它已被证明是一种特殊的科学模型，符合替代、减少和优化原则（3R 原则）。作为三种禽类胚胎外膜之一，CAM 负责胎儿的呼吸、代谢和保护。该模型提供了独特的免疫、血管和细胞外特性组合，同时具有经济实惠和可靠的特点。它可用于癌症生物学、血管生成学、病毒学和毒理学等领域的研究，最近也用于生物化学、药物研究和干细胞生物学。干细胞，特别是源自脂肪组织的间充质干细胞（ADSCs），是组织再生和个性化医疗领域新兴的治疗策略的主题。由于其易于获取、分化特征、免疫调节特性和细胞因子谱，ADSCs 已在上述文件中确立了不同的临床前应用。在这篇综述中，我们旨在强调和确定 CAM 模型在 ADSC 研究中的一些交叉点，重点是伤口愈合和组织工程，以及肿瘤学研究，例如肉瘤。在此，重点在于将这些交叉点的现有证据和经验与 CAM 模型的潜在利用相结合，以进一步研究 ADSC。
