Drink Plant Research Institute/Tea Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, PR China.
Oncol Rep. 2013 Aug;30(2):763-72. doi: 10.3892/or.2013.2504. Epub 2013 May 28.
Cancer of the prostate gland is the most common invasive malignancy and the second leading cause of cancer-related death in human males. Many studies have shown that black tea reduces the risk of several types of cancer. We studied the effects of active extracts of black tea and the black tea polyphenols theaflavins (TFs), on the cellular proliferation and mitochondria of the human prostate cancer cell line PC-3. Our studies revealed that Yinghong black tea extracts (YBT), Assam black tea extracts (ABT) and TFs inhibited cell proliferation in a dose-dependent manner. We also showed that TFs, YBT and ABT affected the morphology of PC-3 cells and induced apoptosis or even necrosis in PC-3 cells. In addition, it was observed that the samples significantly caused loss of the mitochondrial membrane potential, release of cytochrome c from the intermembrane space into the cytosol, decrease of the ATP content and activation of caspase-3 compared with the control. Taken together, these findings suggest that black tea could act as an effective anti-proliferative agent in PC-3 cells, and TFs, YBT and ABT induced apoptosis of PC-3 cells through mitochondrial dysfunction.
前列腺癌是最常见的侵袭性恶性肿瘤，也是男性癌症相关死亡的第二大主要原因。许多研究表明，红茶能降低多种癌症的风险。我们研究了红茶活性提取物和红茶多酚茶黄素（TFs）对人前列腺癌细胞系 PC-3 的细胞增殖和线粒体的影响。研究结果表明，英红红茶提取物（YBT）、阿萨姆红茶提取物（ABT）和 TFs 呈剂量依赖性地抑制细胞增殖。此外，我们还发现 TFs、YBT 和 ABT 影响 PC-3 细胞的形态，并诱导 PC-3 细胞凋亡甚至坏死。此外，与对照组相比，观察到样品显著导致线粒体膜电位丧失、细胞色素 c 从膜间空间释放到细胞质、ATP 含量减少和 caspase-3 激活。综上所述，这些发现表明红茶可能在 PC-3 细胞中作为一种有效的抗增殖剂，TFs、YBT 和 ABT 通过线粒体功能障碍诱导 PC-3 细胞凋亡。
