Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan.
Antimicrob Agents Chemother. 2013 Aug;57(8):4050-2. doi: 10.1128/AAC.00252-13. Epub 2013 May 28.
The efficacies of many antimicrobial peptides are greatly reduced under high salt concentrations, therefore limiting their use as pharmaceutical agents. Here, we describe a strategy to boost salt resistance and serum stability of short antimicrobial peptides by adding the nonnatural bulky amino acid β-naphthylalanine to their termini. The activities of the short salt-sensitive tryptophan-rich peptide S1 were diminished at high salt concentrations, whereas the activities of its β-naphthylalanine end-tagged variants were less affected.
许多抗菌肽在高盐浓度下的功效大大降低，因此限制了它们作为药物的应用。在这里，我们描述了一种通过在其末端添加非天然大体积氨基酸β-萘丙氨酸来提高短抗菌肽的耐盐性和血清稳定性的策略。富含色氨酸的短盐敏感肽 S1 的活性在高盐浓度下减弱，而其β-萘丙氨酸末端标记变体的活性受影响较小。
