Gabrani Reema, Jain Ramya, Sharma Anjali, Sarethy Indira P, Dang Shweta, Gupta S
Department of Biotechnology, Jaypee Institute of Information Technology, Noida-201 307, India.
Indian J Pharm Sci. 2012 Sep;74(5):451-3. doi: 10.4103/0250-474X.108421.
Solanum nigrum is used in various traditional medical systems for antiproliferative, antiinflammatory, antiseizure and hepatoprotective activities. We have evaluated organic solvent and aqueous extracts obtained from berries of Solanum nigrum for antiproliferative activity on leukemic cell lines, Jurkat and HL-60 (Human promyelocytic leukemia cells). The cell viability after the treatment with Solanum nigrum extract was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Results indicated increased cytotoxicity with increasing extract concentrations. Comparative analysis indicated that 50% inhibitory concentration value of methanol extract is the lowest on both cell lines.
龙葵在各种传统医学体系中用于抗增殖、抗炎、抗惊厥和保肝活性。我们评估了从龙葵浆果中获得的有机溶剂提取物和水提取物对白血病细胞系Jurkat和HL - 60（人早幼粒细胞白血病细胞）的抗增殖活性。用MTT（3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基溴化四氮唑）法测定用龙葵提取物处理后的细胞活力。结果表明，随着提取物浓度的增加，细胞毒性增加。比较分析表明，甲醇提取物对两种细胞系的50%抑制浓度值最低。
