College of Environmental & Chemical Engineering, Yanshan University, Qinhuangdao, Hebei province 066004, PR China.
Phytother Res. 2009 Nov;23(11):1524-30. doi: 10.1002/ptr.2769.
We have examined the effects of the crude polysaccharides isolated from Solanum nigrum Linne (SNL-P) in vitro and in vivo against U14 cervical cancer. SNL-P showed no antiproliferative effects in vitro at a dose up to 1 mg/ml. In vivo administration with SNL-P (90, 180, 360 mg/kg b.w., p.o.) decreased the number of ascites tumor cells and prolonged the survival time of U14 cervical-cancer-bearing mice. FACScan flow cytometer analysis showed that most of the ascites tumor cells were arrested in G2/M phase of cell cycle and the ratio of CD4+/CD8+ peripheral blood T-lymphocyte subpopulations were restored following treatment of SNL-P. Furthermore, the treatment with SNL-P also caused a significant increment in IFN-gamma (p < 0.01, 90, 180 and 360 mg/kg b.w.) and a remarkable decrease in Il-4 (p < 0.01, 90, 180 mg/kg b.w.; p < 0.05, 360 mg/kg b.w.) by the method of ELISA. These data showed that SNL-P possess potent antitumor activity and SNL-P might exert antitumor activity via activation of different immune responses in the host rather than by directly attacking cancer cells on the U14 cervical cancer bearing mice. Thus, SNL-P could be used as an immunomodulator and an anticancer agent.
我们研究了从茄属植物（Solanum nigrum Linne）中分离得到的粗多糖（SNL-P）在体外和体内对 U14 宫颈癌的作用。SNL-P 在高达 1 mg/ml 的剂量下在体外没有显示出抗增殖作用。体内给予 SNL-P（90、180、360 mg/kg b.w.，po）可减少腹水肿瘤细胞的数量并延长 U14 宫颈癌荷瘤小鼠的存活时间。流式细胞仪分析显示，大多数腹水肿瘤细胞被阻滞在细胞周期的 G2/M 期，并且 SNL-P 治疗后恢复了外周血 T 淋巴细胞亚群的 CD4+/CD8+比值。此外，SNL-P 的治疗还导致 IFN-γ（p < 0.01，90、180 和 360 mg/kg b.w.）显著增加，IL-4（p < 0.01，90、180 mg/kg b.w.；p < 0.05，360 mg/kg b.w.）显著减少，ELISA 法。这些数据表明 SNL-P 具有很强的抗肿瘤活性，并且 SNL-P 可能通过激活宿主中的不同免疫反应而不是直接攻击 U14 宫颈癌荷瘤小鼠上的癌细胞来发挥抗肿瘤活性。因此，SNL-P 可作为免疫调节剂和抗癌剂。
