Department of Biophysics, Çukurova University, Faculty of Medicine, Adana, Turkey.
Indian J Pharmacol. 2013 Mar-Apr;45(2):168-73. doi: 10.4103/0253-7613.108306.
The present study was aimed at determining the effective doses of Dexmedetomidine (Dex) involved in amplitude of contraction-force and frequency of uterine rings in pregnancy terms of rats. All experiments involving animal subjects were carried out with the approval of animal care and use Ethical Committee of Cukurova University. Experiments were performed on female Albino-Wistar rats (200-260 g; n = 40).
Uterine rings from pregnant rats were placed in organ bath with Krebs and calcium ion (Ca(2+))-free solutions to record and exposed to serially increasing log10 concentrations of Dex.
In Krebs solution, while Dex caused an increase in the spontaneous contraction-forces in all pregnancy terms of rats in a significant dose-dependent manner, it led to a decrease in contraction-frequency in late-pregnancy term of rats. In Ca(2+)-free, the spontaneous contraction-force decreased in late-pregnancy term and increased in early and middle-pregnancy terms. In addition, while Dex increased the contraction-frequency in early and middle-pregnancy terms, it decreased in late-pregnancy term in a dose-dependent manner.
The data were subjected to one-way analysis of variance. Repeated measures were employed for comparison of several group means through the Tukey post-hoc test (SPSS 10.00 Inc., Chicago, Ill, USA). P < 0.05 was considered statistically significant.
This study suggested that Dex might differently alter the spontaneous contraction-forces and contraction-frequencies of uterine rings in all pregnancy terms of rats in Krebs and Ca(2+)-free solutions.
本研究旨在确定右美托咪定（Dex）在妊娠大鼠子宫环收缩力幅度和频率方面的有效剂量。所有涉及动物实验的实验均获得库鲁瓦大学动物护理和使用伦理委员会的批准。实验在雌性白化 Wistar 大鼠（200-260 g；n = 40）上进行。
将妊娠大鼠的子宫环置于含有 Krebs 和钙离子（Ca（2+））的无钙溶液的器官浴中，以记录并暴露于连续增加的 Dex 的对数 10 浓度。
在 Krebs 溶液中，Dex 以剂量依赖性方式引起所有妊娠大鼠的自发性收缩力显著增加，但导致妊娠晚期大鼠的收缩频率降低。在 Ca（2+）-free 中，自发性收缩力在妊娠晚期下降，在妊娠早期和中期增加。此外，Dex 在妊娠早期和中期增加收缩频率，而在妊娠晚期以剂量依赖性方式降低。
对数据进行单因素方差分析。采用 Tukey 事后检验比较多组平均值的重复测量（SPSS 10.00 Inc.，芝加哥，Ill，USA）。P < 0.05 被认为具有统计学意义。
本研究表明，Dex 可能在 Krebs 和 Ca（2+）-free 溶液中以不同的方式改变妊娠大鼠子宫环的自发性收缩力和收缩频率。
