Division of Molecular Cardiology, Department of Medicine, College of Medicine, Texas A&M Health Science Center, Scott & White, Central Texas Veterans Health Care System, Temple, Texas, United States of America.
PLoS One. 2013 May 23;8(5):e64396. doi: 10.1371/journal.pone.0064396. Print 2013.
MircoRNAs (miRNAs) are small non-coding RNAs that govern the gene expression and, play significant role in the pathogenesis of heart failure. The detection of miRNAs in circulation of pulmonary hypertensive (PH) human subjects remains elusive. In the current study, we determined the pattern of miRNAs of mild-to-severe human PH subjects and, compared them with the control subjects by miRNA array. Blood was obtained using fluoroscopic and waveform guided catheterization from the distal (pulmonary artery) port of the catheter. A total 40 human subjects were included in the study and, the degree of PH was determined by mean pulmonary arterial pressure. Among several miRNAs in the array, we validated 14 miRNAs and, the data were consistent with the array profile. We identified several novel downregulated miRNAs (miR-451, miR-1246) and upregulated miRNAs (miR-23b, miR-130a and miR-191) in the circulation of PH subjects. Our study showed novel set of miRNAs which are dysregulated in PH and, are directly proportional to the degree of PH. These miRNAs may be considered as potential biomarker for early detection of PH.
微小 RNA（miRNAs）是一种小型非编码 RNA，能够调控基因表达，在心力衰竭的发病机制中发挥重要作用。在肺动脉高压（PH）患者的循环中检测到 miRNAs 仍然难以实现。在本研究中，我们通过 miRNA 阵列确定了轻度至重度 PH 患者的 miRNA 模式，并将其与对照组进行了比较。通过荧光透视和波形引导导管从导管的远端（肺动脉）端口采集血液。共有 40 名受试者纳入本研究，通过平均肺动脉压确定 PH 的程度。在阵列中的几种 miRNAs 中，我们验证了 14 种 miRNAs，数据与阵列图谱一致。我们在 PH 患者的循环中发现了几种新的下调 miRNA（miR-451、miR-1246）和上调 miRNA（miR-23b、miR-130a 和 miR-191）。我们的研究显示了一组在 PH 中失调的新型 miRNAs，并且与 PH 的程度呈正相关。这些 miRNA 可以被认为是早期检测 PH 的潜在生物标志物。
