Department of Medical Biology, Faculty of Medicine, Giresun University, Giresun, Turkey.
Department of Cardiology, Faculty of Medicine, Ege University, İzmir, Turkey.
Turk J Med Sci. 2023 Feb;53(1):130-141. doi: 10.55730/1300-0144.5566. Epub 2023 Feb 22.
Pulmonary arterial hypertension (PAH) is characterized by maladaptation of pulmonary vasculature which is leading to right ventricular hypertrophy and heart failure. miRNAs play a crucial role in the regulation of many diseases such as viral infection, cancer, cardiovascular diseases, and pulmonary hypertension (PH). In this study, we aimed to investigate the expression pattern of eight human plasma miRNAs (hsa-miR-21-3p, hsa-miR-143- 3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsamiR-206, hsa-miR-210-3p) in mild-to-severe PH patients and healthy controls.
: miRNAs were extracted from the peripheral plasma of the PH patients (n: 44) and healthy individuals (n: 30) by using the miRNA Isolation Kit. cDNA was synthesized using All in-One First strand cDNA Synthesis Kit. Expression of the human plasma hsa-miR- 21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204- 3p, hsa-miR-206, hsa-miR210-3p, and miRNAs were analyzed by qRT-PCR.
According to our results, in PH patients hsa-miR-21-3p and hsa-miR-143-3p expression levels were decreased by 4.7 and 2.3 times, respectively. No significant changes were detected in hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsamiR-206, and hsa-miR-210-3p expression levels between PH and control groups. In addition, considering the severity of the disease, it was observed that the decrease in miR-138, miR-143, miR-145, miR-190, mir-204, mir-206 and miR-208 expressions was significant in patients with severe PH.
: In the early diagnosis of PAH, hsa-miR-21-3p and especially hsa-miR-143-3p in peripheral plasma can be considered as potential biomarkers.
肺动脉高压（PAH）的特征是肺血管的适应不良，导致右心室肥大和心力衰竭。miRNA 在许多疾病的调节中起着至关重要的作用，如病毒感染、癌症、心血管疾病和肺动脉高压（PH）。在这项研究中，我们旨在研究八种人类血浆 miRNA（hsa-miR-21-3p、hsa-miR-143-3p、hsa-miR-138-5p、hsa-miR-145-3p、hsa-miR-190a、hsa-miR-204-3p、hsamiR-206、hsa-miR-210-3p）在轻度至重度 PH 患者和健康对照者中的表达模式。
通过使用 miRNA 分离试剂盒从 PH 患者（n=44）和健康个体（n=30）的外周血浆中提取 miRNA。使用 All in-One First strand cDNA Synthesis Kit 合成 cDNA。通过 qRT-PCR 分析人类血浆 hsa-miR-21-3p、hsa-miR-143-3p、hsa-miR-138-5p、hsa-miR-145-3p、hsa-miR-190a、hsa-miR-204-3p、hsa-miR-206、hsa-miR210-3p 和 miRNA 的表达。
根据我们的结果，在 PH 患者中，hsa-miR-21-3p 和 hsa-miR-143-3p 的表达水平分别降低了 4.7 倍和 2.3 倍。在 PH 组和对照组之间，hsa-miR-138-5p、hsa-miR-145-3p、hsa-miR-190a、hsa-miR-204-3p、hsamiR-206 和 hsa-miR-210-3p 的表达水平没有显著变化。此外，考虑到疾病的严重程度，在严重 PH 患者中，miR-138、miR-143、miR-145、miR-190、mir-204、mir-206 和 mir-208 的表达降低具有显著性。
在 PAH 的早期诊断中，外周血浆中的 hsa-miR-21-3p 特别是 hsa-miR-143-3p 可以作为潜在的生物标志物。
