Vallier P, Bata J, Colobert L
Ann Microbiol (Paris). 1977 Oct;128(3):359-71.
The alpha-amylase secretion in a mineral culture medium containing starch and glucose follow the lysis of mycelium. This lysis seems to result from the hydrolysing action of dextranase and levulanase on cell wall. Cell lysis and amylase secretion are greatly enhanced by pH elevation of culture medium (optimal pH 8,8). In such conditions of production the amylase is not stable but can be stabilized by addition of starch. A method is described using pH and starch content modifications, which allows to obtain an amylase production three times greater than in standard culture medium.
在含有淀粉和葡萄糖的矿物质培养基中，α-淀粉酶的分泌伴随着菌丝体的裂解。这种裂解似乎是由葡聚糖酶和聚酮糖酶对细胞壁的水解作用导致的。培养基pH值升高（最适pH 8.8）可大大增强细胞裂解和淀粉酶分泌。在这种生产条件下，淀粉酶不稳定，但可通过添加淀粉使其稳定。本文描述了一种利用pH值和淀粉含量变化的方法，该方法可使淀粉酶产量比标准培养基中的产量高出三倍。
