[α-淀粉酶合成增加的突变体的产生]
[Production of mutants with an increased alpha-amylase synthesis].
作者信息
Ostrikova N A, Konovalov S A
出版信息
Mikrobiologiia. 1978 Jul-Aug;47(4):722-7.
A mutant characterized by elevated biosynthesis of alpha-amylase was obtained as a result of a three-stage induced selection using nitroso compounds. Changes of mutagens in the course of selection stages and the establishment of their effective doses causing the maximum accumulation of mutations yielded the mutant which produced 2.5 times more alpha-amylase than the parent strain of Aspergillus oryzae 762. The induced variability of the mutant can be registered on a solid growth medium and provides the high activity of alpha-amylase.
通过使用亚硝基化合物进行三阶段诱导选择，获得了一株以α-淀粉酶生物合成量升高为特征的突变体。在选择阶段过程中诱变剂的变化以及确定导致最大突变积累的有效剂量，产生了一种突变体，其产生的α-淀粉酶比米曲霉762亲本菌株多2.5倍。该突变体的诱导变异性可以在固体生长培养基上检测到，并具有高活性的α-淀粉酶。
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