School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Protoplasma. 2013 Dec;250(6):1231-8. doi: 10.1007/s00709-013-0513-z. Epub 2013 May 30.
As protoplasts have the characteristics of no cell walls, rapid population growth, and synchronicity, they are useful tools for research in many fields, especially cellular biology (Table 1). This article is an overview that focuses on the application of protoplasts to investigate the mechanisms of dedifferentiation, including changes in hormone signals, epigenetic changes, and organelle distribution during the dedifferentiation process. The article also emphasizes the wide range of uses for protoplasts in studying protein positions and signaling during different stresses. The examples provided help to show that protoplast systems, for example the mesophyll protoplast system of Arabidopsis, represent promising tools for studying developmental biology. Meanwhile, specific analysis of protoplast, which comes from different tissue, has specific advantages and limitations (Table 2), and it can provide recommendations to use this system.
由于原生质体具有无细胞壁、生长迅速和同步性的特点，因此它们是许多领域研究的有用工具，特别是细胞生物学领域（表 1）。本文是一篇综述，重点介绍了原生质体在研究去分化机制中的应用，包括去分化过程中激素信号、表观遗传变化和细胞器分布的变化。文章还强调了原生质体在研究不同胁迫下蛋白质位置和信号的广泛用途。提供的例子表明，原生质体系统，例如拟南芥的叶肉原生质体系统，代表了研究发育生物学的有前途的工具。同时，来自不同组织的原生质体的具体分析具有特定的优势和局限性（表 2），并可以提供使用该系统的建议。
