Warghat Ashish R, Thakur Kanika, Sood Archit
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur, Himachal Pradesh, 176061, India.
Mol Biol Rep. 2018 Dec;45(6):2897-2905. doi: 10.1007/s11033-018-4344-z. Epub 2018 Sep 8.
Plant stem cell research is of interest due to stem cells ability of unlimited division, therapeutic potential and steady supply to provide precursor cells. Their isolation and culture provides the important source for the production of homogenous lines of active constituents that allow large-scale production of various metabolites. The process of dedifferentiation and reversal to pluripotent cells involves the various pathways genes related to the stem cells and are associated to each other for maintaining a specific niche. Domains such as niche dynamics and maintenance signaling can be used for the identification of genes for stem cell niche. Significant findings have been achieved in the past on plant stem cells however our understanding towards mechanisms underlying some specific phenomenon like dedifferentiation, regulation, niche dynamics is still in infancy. The present review is based on the past research efforts and also pave a way forward for the future anticipation in the field of development of cell cultures for the production of active metabolites on large scale and undertanding transcriptional regulation of stem cell genes involved in niche signaling.
植物干细胞研究备受关注，因为干细胞具有无限分裂能力、治疗潜力以及能够稳定供应前体细胞。它们的分离和培养为生产活性成分的同质细胞系提供了重要来源，从而能够大规模生产各种代谢产物。去分化和逆转为多能细胞的过程涉及与干细胞相关的各种信号通路基因，这些基因相互关联以维持特定的生态位。生态位动态和维持信号等领域可用于鉴定干细胞生态位的基因。过去在植物干细胞方面已经取得了重大发现，然而我们对一些特定现象（如去分化、调控、生态位动态）背后机制的理解仍处于起步阶段。本综述基于过去的研究成果，也为未来大规模生产活性代谢产物的细胞培养发展领域以及理解参与生态位信号传导的干细胞基因的转录调控铺平了道路。
