Kosaka Nobuyoshi, Yoshioka Yusuke, Hagiwara Keitaro, Tominaga Naoomi, Ochiya Takahiro
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan.
Methods Mol Biol. 2013;1024:1-10. doi: 10.1007/978-1-62703-453-1_1.
Circulating microRNAs (miRNAs), also known as secretory miRNAs, are packaged in small membrane vesicles called exosomes. These exosomal miRNAs are secreted from various cell types and incorporated inside the recipient cells. The functions of exosomal miRNAs are poorly understood, but some reports have shown their essential roles in cancer development. Therefore, methods to study the function of exosomal miRNAs not only in vitro but also in vivo might be essential. We have analyzed the function of exosomal miRNAs by miRNA-enriched exosomes both in vitro and in vivo. In this chapter, the methods to concentrate the targeted miRNAs are provided. This simple and useful method enables the study of the precise mechanisms of exosomal miRNAs under physiological and pathological conditions.
循环微RNA（miRNA），也称为分泌性miRNA，被包裹在称为外泌体的小膜泡中。这些外泌体miRNA从各种细胞类型中分泌出来，并被纳入受体细胞内。外泌体miRNA的功能尚不清楚，但一些报告显示它们在癌症发展中起着重要作用。因此，不仅在体外而且在体内研究外泌体miRNA功能的方法可能至关重要。我们已经通过富含miRNA的外泌体在体外和体内分析了外泌体miRNA的功能。在本章中，提供了富集靶向miRNA的方法。这种简单而有用的方法能够在生理和病理条件下研究外泌体miRNA的精确机制。
