Jackson R M, Veal C F, Beckman J S, Brannen A L
Birmingham VA Medical Center, Alabama.
Am J Med Sci. 1990 Jul;300(1):22-8. doi: 10.1097/00000441-199007000-00005.
This study evaluated the effects of polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) in re-expansion pulmonary edema, a unilateral lung injury due in part to re-oxygenation of hypoxic, collapsed lung tissue. The hypothesis underlying this investigation was that extracellular superoxide contributed to the lung inflammation in this model, and that PEG-SOD could be used to test for extra-cellular superoxide involvement. The right lungs of 2-3 kg rabbits were collapsed for seven days by intrapleural air injections. Immediately prior to lung re-expansion, rabbits received intravenously 10,000 units/kg PEG-SOD (n = 6) or an equal volume of H2O2-inactivated PEG-SOD (n = 6). Inactive PEG-SOD pretreated rabbits had a marked increase in re-expanded lungs' lavage albumin concentration (right 1653 +/- 230 micrograms/ml, left 404 +/- 160 micrograms/ml; p less than .01). Active PEG-SOD did not inhibit this permeability increase (right 1744 +/- 242 micrograms/ml, left 180 +/- 53 micrograms/ml; p less than .01). However, active PEG-SOD significantly decreased both total number and percent neutrophils in alveolar lavage (right 24.8 +/- 9.4%, left 4.2 +/- 0.8%; p less than .05) compared to inactive PEG-SOD pretreated rabbits (right 52.8 +/- 5.8%, left 8.7 +/- 2.4%; p less than .01). Pretreatment with active PEG-SOD significantly increased lung tissue (20.4 +/- 1.5 units/mg DNA), blood (400 +/- 8 units/ml) and right lung lavage (30.0 +/- 3.1 units/ml) SOD activities compared to those from inactive PEG-SOD pretreated rabbits (respectively: 16.0 +/- 1.0 units/mg DNA, 335 +/- 14 units/ml and 10.8 +/- 1.3 units/ml; p less than .05 for each comparison).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究评估了聚乙二醇共轭超氧化物歧化酶（PEG-SOD）在复张性肺水肿中的作用，复张性肺水肿是一种单侧肺损伤，部分原因是缺氧、萎陷的肺组织重新充氧。本研究的假设是，细胞外超氧化物在该模型中导致肺部炎症，且PEG-SOD可用于检测细胞外超氧化物的参与情况。通过胸腔内注射空气，使2-3千克兔子的右肺萎陷7天。在肺重新扩张前，兔子静脉注射10000单位/千克PEG-SOD（n = 6）或等体积的过氧化氢灭活的PEG-SOD（n = 6）。用灭活PEG-SOD预处理的兔子，重新扩张肺的灌洗白蛋白浓度显著增加（右肺1653±230微克/毫升，左肺404±160微克/毫升；p＜0.01）。活性PEG-SOD并未抑制这种通透性增加（右肺1744±242微克/毫升，左肺180±53微克/毫升；p＜0.01）。然而，与用灭活PEG-SOD预处理的兔子相比（右肺52.8±5.8%，左肺8.7±2.4%；p＜0.01），活性PEG-SOD显著降低了肺泡灌洗中中性粒细胞的总数和百分比（右肺24.8±9.4%，左肺4.2±0.8%；p＜0.05）。与用灭活PEG-SOD预处理的兔子相比（分别为：16.0±1.0单位/毫克DNA、335±14单位/毫升和10.8±1.3单位/毫升；每次比较p＜0.05），用活性PEG-SOD预处理显著提高了肺组织（20.4±1.5单位/毫克DNA）、血液（400±8单位/毫升）和右肺灌洗（30.0±3.1单位/毫升）的SOD活性。（摘要截取自250词）
