Pennings Gabrielle J, Siegwald Justine, Yong Andy S C, Kritharides Leonard, Lowe Harry C
Anzac Research Institute, University of Sydney, Sydney, NSW, Australia.
J Thromb Thrombolysis. 2014 Apr;37(3):326-30. doi: 10.1007/s11239-013-0948-2.
Platelets and leukocytes play an important role in atherosclerotic plaque progression. Determining the activation state of both the platelet and leukocyte population at the lesion site has become increasingly of interest. A novel thrombus aspiration catheter, the Thrombuster II (Kaneka Medical Products, Osaka, Japan), has recently been introduced into clinical practice, and is finding rapidly increasing use. This catheter is capable of local blood collection within the coronary tree, but to our knowledge has not previously been validated to demonstrate lack of platelet activation. This study therefore aims to establish whether or not blood sampling via the Thrombuster II results in artefactual platelet activation. Duplicate blood samples were obtained from the descending aorta using both the Thrombuster II and a femoral arterial sheath (control). The samples were collected into CTAD (to minimize ex vivo activation) blood collection tubes and processed for flow cytometry analysis. Platelet activation was assessed based on the expression of CD62P, PAC-1 binding and platelet CD147 expression. Platelet-leukocyte aggregates were also examined. No significant difference was noted between the sheath samples and the Thrombuster II catheter. The Thrombuster II catheter is a novel thrombus aspiration device capable of providing the assessment of platelet activation and platelet-leukocyte aggregates in coronary arteries.
血小板和白细胞在动脉粥样硬化斑块进展中起重要作用。确定病变部位血小板和白细胞群体的激活状态越来越受到关注。一种新型血栓抽吸导管——血栓清除器II（日本大阪Kaneka医疗产品公司）最近已应用于临床实践，且使用量迅速增加。该导管能够在冠状动脉内进行局部采血，但据我们所知，此前尚未经过验证以证明不会导致血小板激活。因此，本研究旨在确定通过血栓清除器II采集血液是否会导致人为的血小板激活。使用血栓清除器II和股动脉鞘管（对照）从降主动脉采集两份重复的血液样本。将样本收集到CTAD（以尽量减少体外激活）采血管中，并进行流式细胞术分析。基于CD62P的表达、PAC-1结合和血小板CD147表达评估血小板激活情况。还检查了血小板-白细胞聚集体。鞘管样本和血栓清除器II导管之间未观察到显著差异。血栓清除器II导管是一种新型血栓抽吸装置，能够评估冠状动脉中的血小板激活和血小板-白细胞聚集体。
