Bailly A L, Laurent A, Lu H, Elalami I, Jacob P, Mundler O, Merland J J, Lautier A, Soria J, Soria C
Laboratory of Neuroradiology and Therapeutic Angiography, Faculté de Médecine Lariboisière-St Louis, Paris, France.
J Biomed Mater Res. 1996 Jan;30(1):101-8. doi: 10.1002/(SICI)1097-4636(199601)30:1<101::AID-JBM13>3.0.CO;2-R.
One of the major problems in the use of catheters is their thrombogenicity, since the embolization of clots near the central nervous system or the coronary arteries can cause permanent damage. In this work we have compared the in vivo thrombogenicity of four different angiography catheters and their in vitro activation of fibrinogen binding and platelet retention. The thrombogenicity of catheters has been evaluated in angiographic conditions by kinetic evaluation of the reduction of blood flow rate through the catheters. The binding of adhesive proteins (fibrinogen and von Willebrand factor [vWF] was studied in vitro using a direct-ELISA technique after circulation of anticoagulated whole blood through sections of catheters. The retention of platelets was studied in vitro using 111Indium-labelled platelets. Fibrinogen binding and platelet retention both seem to be good predictors of catheter thrombogenicity, fibrinogen being the better of the two. The most thrombogenic material has the highest fibrinogen and platelet retention rate. This study also confirms the inefficiency of albumin precoating for the prevention of fibrinogen deposition. The determination of fibrinogen deposition by direct-ELISA technique and platelet retention rate is very useful for preclinical testing of catheters.
使用导管的主要问题之一是其血栓形成性，因为中枢神经系统或冠状动脉附近的血栓栓塞会导致永久性损伤。在这项工作中，我们比较了四种不同血管造影导管的体内血栓形成性及其体外纤维蛋白原结合和血小板滞留的激活情况。通过对导管内血流速度降低的动力学评估，在血管造影条件下评估了导管的血栓形成性。在抗凝全血通过导管段循环后，使用直接酶联免疫吸附测定（ELISA）技术在体外研究了粘附蛋白（纤维蛋白原和血管性血友病因子[vWF]）的结合情况。使用111铟标记的血小板在体外研究了血小板的滞留情况。纤维蛋白原结合和血小板滞留似乎都是导管血栓形成性的良好预测指标，两者中纤维蛋白原的预测效果更好。血栓形成性最强的材料具有最高的纤维蛋白原和血小板滞留率。这项研究还证实了白蛋白预涂层预防纤维蛋白原沉积的无效性。通过直接ELISA技术测定纤维蛋白原沉积和血小板滞留率对于导管的临床前测试非常有用。
