Wang Caroline C, Hartmann-Fischbach Petra, Krueger Tim R, Wells Terry L, Feineman Amy R, Compton Joanne C
Industrial Laboratories, 4046 Youngfield Street, Wheat Ridge, CO, USA.
Drug Test Anal. 2014 Apr;6(4):342-9. doi: 10.1002/dta.1487. Epub 2013 May 29.
Dermorphin and HYP(6) -dermorphin are hepta-peptides and natural opioids originally isolated from the skin of South American frogs. They are more potent than morphine but less likely to produce drug tolerance and addiction. These properties make them ideal candidates for the doping of racehorses to enhance performance during competition. Dermorphin was recently classified as a Class I drug by Racing Commissioners International (RCI), indicating that it is a banned substance in equine athletes. To enforce this ban, a fast and sensitive method was developed for dermorphin and HYP(6)-dermorphin analysis in equine plasma. Equine plasma (2 ml) was extracted on a mixed mode cation exchange solid-phase column. After extraction, dermorphin and HYP(6)-dermorphin were separated and detected using a liquid chromatography (LC) triple quadrupole linear ion trap mass spectrometry in positive multiple-reaction-monitoring (MRM) mode. Each analysis was 3.5 min. Four MRM transitions were used for identification of each compound. The extraction procedure was efficient and the limits of detection (LOD) were 2 pg/ml and 10 pg/ml for dermorphin and HYP(6)-dermorphin, respectively. The method has good selectivity and precision. Results of stability studies showed that both analytes were stable at low temperature. This is the first report of dermorphin and HYP(6)-dermorphin analysis in equine plasma, which could be adopted as a regular screening or confirmation method for controlling the abuse of these compounds in equine sports.
德莫啡肽和HYP(6)-德莫啡肽是七肽类天然阿片样物质，最初是从南美青蛙的皮肤中分离出来的。它们比吗啡更有效，但产生药物耐受性和成瘾的可能性较小。这些特性使它们成为赛马比赛中用于提高成绩的理想兴奋剂候选物。德莫啡肽最近被国际赛马委员会（RCI）列为一类药物，这表明它在马匹运动员中是被禁止的物质。为了执行这一禁令，开发了一种快速灵敏的方法用于马血浆中德莫啡肽和HYP(6)-德莫啡肽的分析。取2毫升马血浆在混合模式阳离子交换固相柱上进行萃取。萃取后，使用液相色谱（LC）三重四极杆线性离子阱质谱仪在正离子多反应监测（MRM）模式下对德莫啡肽和HYP(6)-德莫啡肽进行分离和检测。每次分析用时3.5分钟。每种化合物的鉴定使用四个MRM跃迁。萃取过程高效，德莫啡肽和HYP(6)-德莫啡肽的检测限分别为2皮克/毫升和10皮克/毫升。该方法具有良好的选择性和精密度。稳定性研究结果表明，两种分析物在低温下均稳定。这是关于马血浆中德莫啡肽和HYP(6)-德莫啡肽分析的首次报道，可作为控制这些化合物在马术运动中滥用的常规筛查或确证方法。
