Richards Stacey L, Cawley Adam T, Raftery Mark J
Australian Racing Forensic Laboratory, Racing NSW, Sydney, NSW, Australia.
Bioanalysis. 2013 Dec;5(24):2995-3007. doi: 10.4155/bio.13.281.
Dermorphin, a hepta-peptide with potent analgesic properties, is classified as a doping agent in equine racing. Since its discovery, a number of biologically active structural analogs have been synthesized and made commercially available so there is a need for reliable methods of detection.
METHODOLOGY/RESULTS: A sensitive detection method was developed for dermorphin and six analogs in equine urine. Peptide enrichment was achieved using weak cation exchange with subsequent separation and detection by nano-UHPLC-MS/MS. Method validation parameters included: specificity, linearity (5-10000 pg/ml), recovery (58-93%), intra and inter-assay repeatability, LOD (5-50 pg/ml) and matrix effects.
The presented method will facilitate the control of the abuse of dermorphin and selected analogs in equine sports.
Dermorphin是一种具有强效镇痛特性的七肽，在赛马运动中被列为兴奋剂。自其被发现以来，已合成了许多具有生物活性的结构类似物并投入商业使用，因此需要可靠的检测方法。
方法/结果：开发了一种用于检测马尿中Dermorphin及其六种类似物的灵敏检测方法。使用弱阳离子交换实现肽富集，随后通过纳米超高效液相色谱-串联质谱进行分离和检测。方法验证参数包括：特异性、线性（5-10000 pg/ml）、回收率（58-93%）、批内和批间重复性、检测限（5-50 pg/ml）和基质效应。
所提出的方法将有助于控制马类运动中Dermorphin及选定类似物的滥用。
